主要成就
主要從事微生物技術(shù)在農(nóng)產(chǎn)品安全生產(chǎn)中的應(yīng)用方面的研究。作為主要貢獻(xiàn)人參與了農(nóng)藥殘留微生物降解技術(shù)的研發(fā)，建立了國(guó)內(nèi)最大的農(nóng)藥殘留降解微生物菌種資源庫(kù)，提出了農(nóng)藥殘留微生物降解田間應(yīng)用標(biāo)準(zhǔn)，并在水稻、蔬菜和茶葉等農(nóng)作物上進(jìn)行了大規(guī)模的試驗(yàn)示范，項(xiàng)目獲得2005年度國(guó)家科技進(jìn)步二等獎(jiǎng)。近年來(lái)開(kāi)展了新型糖苷水解酶資源開(kāi)發(fā)以及生防菌粘細(xì)菌在植物病害防治方面的研究和應(yīng)用等工作，尤其是生防粘細(xì)菌EGB在枯萎病和稻瘟病防治方面取得了良好的研究成果。先后主持完成國(guó)家“863”計(jì)劃項(xiàng)目、國(guó)家自然科學(xué)基金項(xiàng)目多項(xiàng)。2005-2007年在日本京都大學(xué)進(jìn)行了蛋白質(zhì)晶體結(jié)構(gòu)解析方面的研究工作，熟悉蛋白質(zhì)晶體培養(yǎng)、X-衍射數(shù)據(jù)分析析，解析了海藻糖降解途徑中的鼠李糖苷酶的結(jié)構(gòu)。發(fā)表論文100余篇，其中在Appl. Environ. Microbiol.,Appl Microbiol Biotech.,Biol Control等雜志上發(fā)表SCI論文50余篇。
人物履歷
2006/06-至今，南京農(nóng)業(yè)大學(xué)，生命科學(xué)學(xué)院，教授2005/10-2007/02，日本國(guó)京都大學(xué)，農(nóng)學(xué)部，博士后，合作導(dǎo)師 Kousaku Murata2003/05-2005/08，中國(guó)科學(xué)院南京土壤研究所，博士后，合作導(dǎo)師趙其國(guó)院士2002/05-2006/06，南京農(nóng)業(yè)大學(xué)，生命科學(xué)學(xué)院，副教授2000/08-2002/05，南京農(nóng)業(yè)大學(xué)，資源與環(huán)境學(xué)院，講師研究方向
1. 生物降解與生物轉(zhuǎn)化; BiodegradationandBioconversion.2. 酶資源與蛋白質(zhì)工程;Enzyme resources and protein engineering3. 土傳病害真菌防治; Biocontrol ofplant fungal disease發(fā)表論文
1. Jie Zhou, Zhoukun Li, Han Zhang, Jiale Wu, Xianfeng Ye, Weiliang Dong, Min Jiang, Yan Huang,Zhongli Cui*, A Novel Maltogenic Amylase CoMA fromCorallococcussp. EGB Catalyzes the Conversion of Maltooligosaccharidesand Soluble Starch to Maltose.Appl Environ Microb, 2018.2. Qiongzhen Chen; Yajuan Duan; Zhoukun Li;Zhongli Cui*; Weidong Liu, Yan Huang*. Crystal structure of p-nitrophenol 4-monooxygenase PnpA fromPseudomonas putidaDLL-E4: the key enzyme involved in p-nitrophenol degradation.Biochemical and Biophysical Research Communications, BBRC. 20183. Zhoukun Li #,Ting Wang #, Xue Luo, Xiangmin Li, Chengyao Xia, Yuqiang Zhao, Xianfeng Ye, Yan Huang, Xiangyang Gu, Hui Cao,Zhongli Cui*, Jiaqin Fan *. Biocontrol potential ofMyxococcussp. strain BS against bacterial soft rot of calla lily caused byPectobacterium carotovorum.Biol Control, 2018, 126: 36-444. Zhoukun Li, Xianfeng Ye, Peilin Chen, Kai Ji, Jie Zhou, Fei Wang, Weiliang Dong, Yan Huang, Zhengguang Zhang,Zhongli Cui*, Antifungal potential ofCorallococcussp. strain EGB against plant pathogenic fungi,Biol Control, 2017, 110: 10-175. Jie Zhou#, Zhoukun Li#, Jiale Wu, Lifeng Li, Ding Li, Xianfeng Ye, Xue Luo, Yan Huang,Zhongli Cui*, Hui Cao*, Functional analysis of a novel β-(1,3)-glucanase fromCorallococcussp. EGB containing a fascin-like module,Appl Environ Microb, 2017, 83(16): e01016-176. Weiliang Dong#, Fei Wang#, Fei Huang#, Yicheng Wang, Jie Zhou, Xianfeng Ye, Zhoukun Li, Yin Hou, Yan Huang, Jiangfeng Ma, Ming Jiang,Zhongli Cui*. Metabolic pathway involved in 6-chloro-2-benzoxazolinone degradation byPigmentiphagasp. strain DL-8 and identification of the novel metal-dependent hydrolase CbaA.Appl. Environ. Microbiol, 2016, 82(14): 4169-41797. Zhoukun Li, Jiale Wu, Biying Zhang, Fei Wang, Xianfeng Ye, Yan Huang, Qiang Huang,Zhongli Cui*. AmyM, a novel maltohexaose-forming α-amylase fromCorallococcussp. strain EGB.Appl. Environ. Microbiol, 2015, 81(6): 1977-19878. Fei Wang, Jie Zhou, Zhoukun Li, Weiliang Dong, Ying Hou, Yan Huang,Zhongli Cui*. Involvement of the cytochrome P450 system EthBAD in the N-deethoxymethylation of acetochlor byRhodococcussp. strain T3-1.Appl. Environ. Microbiol, 2015, 81(6): 2182-21889. Weiliang Dong#, Qiongzhen Chen#, Ying Hou, Shuhuan Li, Kai Zhuang, Fei Huang, Jie Zhou, Zhoukun Li, Jue Wang, Lei Fu, Zhengguang Zhang, Yan Huang, Fei Wang,Zhongli Cui*. Metabolic Pathway Involved in 2-Methyl-6-Ethylaniline Degradation bySphingobiumsp. Strain MEA3-1 and Cloning of the Novel Flavin-Dependent Monooxygenase System meaBA.Appl. Environ. Microbiol, 2015, 81(24): 8254-826410. Fei Wang, Ying Hou, Jie Zhou, Zhoukun Li, Yan Huang,Zhongli Cui*. Purification of an amide hydrolase DamH fromDelftiasp. T3-6 and its gene cloning, expression, and biochemical characterization.Appl Microbiol Biotech, 2014, 98(17): 7491-749911. Jingquan Li#, Yan Huang#, Ying Hou, Xiangmin Li, Hui Cao,Zhongli Cui*. Novel Gene Clusters and Metabolic Pathway Involved in 3,5,6-Trichloro-2-Pyridinol Degradation byRalstoniasp.T6.Appl. Environ. Microbiol. 2013, 79(23):7445-5312.Ying Hou, Jian Tao, Wenjing Shen, Juan Liu, Jingquan Li, Yongfeng Li, Hui Cao &Zhongli Cui. Isolation of the fenoxaprop-ethyl (FE)-degrading bacteriumRhodococcussp. T1, and cloning of FE hydrolase genefeh.FEMS Microbiol Lett. 2011,323(2):196-203.榮譽(yù)記錄
教育部技術(shù)發(fā)明獎(jiǎng)，土壤農(nóng)藥污染微生物修復(fù)技術(shù)的研究與應(yīng)用，二等，2007，第二獲獎(jiǎng)人 (李順鵬，崔中利，何健，洪青，蔣建東，管曉進(jìn))國(guó)家科技進(jìn)步獎(jiǎng)，農(nóng)藥殘留微生物降解技術(shù)的研究與應(yīng)用，二等，2005，第二獲獎(jiǎng)人（10）(李順鵬、崔中利、沈 標(biāo)、劉 智、何 健、楊興明、王新華、張瑞福、蔣建東、洪 青)
