人物經(jīng)歷
教育經(jīng)歷
1986.9 1989.8東北師范大學(xué)植物學(xué)博士
1982.3 1984.8東北師范大學(xué)遺傳學(xué)碩士
1978.3 1982.2遼寧師范大學(xué)生物學(xué)學(xué)士
1969.9 1972.8丹東市第二中學(xué)高中課程
工作經(jīng)歷
1998.3 至今大連理工大學(xué)教師
1984.10 1998.2遼寧師范大學(xué)教師
1975.12 1978.2丹東市陶瓷廠工人
1973.12 1975.12丹東市東溝縣海洋紅公社知青
1972.12 1973.12丹東市鳳城縣賽馬公社知青
社會(huì)兼職
2006.5至今評(píng)委(國(guó)家留學(xué)基金委)
2007.10至今副理事長(zhǎng)(中國(guó)園藝學(xué)會(huì)小漿果分會(huì))
2005.12020.12學(xué)部委員(教育部科委生命科學(xué)學(xué)部)
2005.12020.12理事(中國(guó)遺傳學(xué)會(huì))
2004.122019.12專家(遼寧省生物技術(shù)協(xié)會(huì)專家委員會(huì))
研究領(lǐng)域
1. 植物轉(zhuǎn)基因工程與育種技術(shù)平臺(tái)研究。編號(hào):JY03-B-18,國(guó)家科技部植物轉(zhuǎn)基因重大專項(xiàng),100萬(wàn)元,2003-2004
2. 基因工程關(guān)鍵技術(shù)。項(xiàng)目編號(hào):2001101001,遼寧省科技廳,210萬(wàn)元,2001-2004
3. 無(wú)載體、無(wú)標(biāo)記基因植物轉(zhuǎn)基因技術(shù)。項(xiàng)目編號(hào):2003B4NS015,大連市科技局,100萬(wàn)元,2003-2004
4. 蛇島蝮蛇類(lèi)凝血酶基因的分離和表達(dá)。遼寧省科技攻關(guān),10萬(wàn)元,1999.01-2001.12
5. 蛇島蝮蛇類(lèi)凝血酶基因的分離和表達(dá)。大連市科委,15萬(wàn)元,1998-2001
6. 胸腺素α1基因工程藥物開(kāi)發(fā)。通化金馬制藥股份公司,390萬(wàn)元,1998.01~2001.12
7. 楊樹(shù)基因工程育種。鞍山生生高新植物技術(shù)應(yīng)用股份有限公司,25萬(wàn)元,2001.9-2003.9
8. 長(zhǎng)春花堿單體的分離研究。大連理工大學(xué)領(lǐng)先集團(tuán)有限公司,20萬(wàn)元,2002.7-2003.7
9. 真?当=」πУ尿(yàn)證與鑒定。大連理工大學(xué)領(lǐng)先集團(tuán)有限公司,10萬(wàn)元,2002.7-2003.7
指導(dǎo)碩、博士生研究方向:植物基因工程、生物制藥
出版著作
五年來(lái)共發(fā)表文章120余篇,SCI收錄42篇,主要文章包括:
1. Properties of A. ficuum AS3.324 phytase expressed in tobacco. Process Biochemistry,2005,40:213u2013216 SCI
2. Purification,characterization and gene cloning of a novel phospholipase A2 from the venom of Agkistrodon blomhoffii ussurensis. The International Journal of Biochemical & Cell Biology. 2005,37:558-565 SCI
3. Neuroprotective properties of catalpol in transient global cerebral ischemia in gerbils: dose-response,therapeutic time-window and long-term efficacy. Brain Research,2004,1029:179-185 SCI
4. Plasmodium falciparum histone acetyltransferase,a yeast GCN5 homologue involved in chromatin remodeling. Eukaryotic Cell,2004,3(2):264-276
5. Catalpol inhibits apoptosis in hydrogen peroxide-induced PC12 cells by preventing cytochrome c release and inactivating of caspase casecade. Toxicon,2004,43:53-59
6. PfADA2,a Plasmodium falciparum homologue of the transcriptional coactivator ADA2 and its in vivo association with the histone acetyltransferase PfGCN5. Gene,2004,336:251-261
7. Construction of Targeting Vector and Expression of Green Fluorescent Protein in the Silkworm,Antheraea pernyi. DNA and Cell Biology. 2003,22:441-446
8. The Expression of GFP Under the Control of Fibroin Promotor in Primary ovarian cells of Antheraea pernyi. Journal of Biosciences,2003,28(6):691-695
9. 菟絲子提取物在PC12細(xì)胞株中的神經(jīng)營(yíng)養(yǎng)因子樣活性。生物化學(xué)與生物物理進(jìn)展。2003,30(2):226-230
10. Codonopsis pilosula (Franch) Nannf total alkaloids potentiate neurite outgrowth induced by nerve growth factor in PC12 cells. Acta Pharmacologica Sinica,2003,24(9):913-917
11. Effect of Cuscuta chinensis glycoside on the neuronal differentiation of rat pheochromocytoma PC12 cells. International Journal of Developmental Neuroscience,2003,21:277-281
12. Hydrogen peroxide-induced apoptosis in PC12 cells and the protective effect of puerarin. Cell Biology International,2003,27:1025-1031
13. Neuroprotective effect of Alpinia oxyphylla Miq. fruits against glutamate-induced apoptosis in cortical neurons. Toxicology Letters,2003,144(2):205u2013212
14. Neuroprotective effect of Alpinia oxyphylla extract against glutamate excitotoxicity in cultured mouse cortical neurons. Neurosci Res Commun,2003,33(2):105u2013113
15. Investigation of the neuronal death mode induced by glutamate treatment in serum-,antioxidant-free primary cultured cortical neurons. Developmental Brain Research,2003,145(2):263-268
16. A new way for chemical degradation of plastic by natural volatile constituents of Ledum palustre. Chinese Science Bulletin,2003,48(16):1718-1721
17. Enhancing ethanol tolerance of a self-flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae by Mg2+ via reduction in plasma membrane permeability. Biotechnology Letters,2003,25:1191-1194
18. Dextromethorphan protects dopaminergic neurons against inflammation-mediated degeneration through inhibition of microglial activation. The Journal Of Pharmacology and Experimental Therapeutics,2003,305(1):212-218
19. 酸性水溶液中硫氰酸鉬(V)配合物的研究。光譜學(xué)與光譜分析,2003,23(2)337-339
20. Purification and characterization of ulva pertusa kjellm alkaline phosphatase. Preparative Biochemistry & Biotechnology,2003,33(2):113-123
21. Cloning of cDNA Encoding Choline Monooxygenase from Suaeda liaotungensis and Salt Tolerance of Transgenic Tobacco. Acta Botanica Sinica,2003,45(2):242-247
22. Molecular cloning and characterization of betaine aldehyde dehydrogenase gene from Suaeda liaotungensis and its use in improved tolerance to salinity in transgenic tobacco. Biotechnol Lett. 2003,25(17):1431-1436
23. Characterization of Sporothric schenckii by random amplication of polymorphic DNA assay. Chinese Medicine Journal,2003,116(2):239-242
24. Expression of gloshedobin,a thrombin-like enzyme from the venom of Gloydius shedaoensis,in Escherichia coli. Biotechnology Letters,2003,25(2):101u2013104
25. High-level expression of a soluble snake venom enzyme,gloshedobin,in E. coli in the presence of metal ions. Biotechnology Letters,2003,25(8):607u2013610
26. Purification and characterization of ginsenoside--arabinofuranase hydrolyzing ginsenoside Rc into Rd from the fresh root of Panax ginseng. Process Biochemistry,2002,37:793-798
27. Cloning and Expression of defibrase cDNA from the venom of Gloydius shedaoensis. Biotechnology Letters,2002,24:135-138
28. Cloning Expression and Purification of Gussrobin A Thrombin-like Enzyme from the Snake Venom of Gloydius ussuriensis. Prog Biochem Biophys. 2002,34(1):6-10
29. Expression,Purification and Partial Characterization of Recombinant Defibrase,a Thrombin-Like Enzyme from the Venom of Glyodius shedaoensis. Prog Biochem Biophys. 2002,29(3)390-393
30. Production of a Functional Single-chain Fv Protein in Transgenic Tobacco Root Exudates. Biotechnology letters. 2002,24(18):1531-1534
31. An expeditious method for constructing T-vectors using Eam 1105 Ⅰ cassettes. Plant Molecular Biology Reporter,2002,20:189a-189e
32. Application of DNA sequencing in detection of telomerase activity. Biochemical Engineering Journal. 2002,11(2-3):79-85
33. Production of high affinity human single-chain antibody against preS1 of Hepatitis B Virus: comparison of large naïve and in vitro immune phage displayed antibody library. Progressing in Biochemistry and Biophysics. 2002,29(4):572-575
34. Inhibition by naloxone stereoisomers of beta-amyloid peptide (1-42)-induced superoxide production in microglia and degeneration of cortical and mesencephalic neurons. Journal of Pharmacology and Experimental Therapeutics,2002,302(3):1212-1219
35. A Serum- and Antioxidant-free Primary Culture Model of Mouse Cortical Neurons for Pharmacological Screen and Studies of Neurotrophic and Neuroprotective Agents. Cellular and Molecular Neurobiology. 2002,22(2):197-206
36. Expression of endoplasmic reticulum molecular chaperon GRP94 in human lung cancer tissues and its clinical significance. Chinese Medical Journal,2002,115(11):1615-1619
37. Embryonic stem cell as nuclear donor could promote in vitro development of the heterogeneous reconstructed embryo. Chinese Science Bulletin,2002,47(21):1811-1815
38. 銅鋅超氧化物歧化酶與色氨酸鈷(Ⅱ)的相互作用。光譜學(xué)與光譜分析,2000,3:305-307
39. 紫外-可見(jiàn)吸收光譜法測(cè)定配合顯色反應(yīng)的焓變。光譜學(xué)與光譜分析,2001,21(4):527-528
40. Kinetics of Inactivation of Ulva pertusa Kjellm Alkaline Phosphatase by Ethylenediaminetetraacetic Acid Disodium. Journal of Enzyme Inhibition,2001,16:313-319
41. Purification and Characterization of ginsenoside-?-Glucosidase from Ginseng. Chem. Pharm. Bull,2001,49(7):795-798
42. 分光光度法研究EDTA對(duì)小牛腸堿性磷酸酶的不可逆抑制動(dòng)力。光譜學(xué)與光譜分析,2001,21(5):701-703
43. 堿性磷酸梅與Cu (Ⅱ) 相互作用的譜學(xué)研究。光譜學(xué)與光譜分析,2001,21(4):432-434
44. 氨基;概cCu(Ⅱ)相互作用的譜學(xué)研究。光譜學(xué)與光譜分析,2001,21(6):804-806
45. 可見(jiàn)光譜學(xué)研究核酸酶P1與氯化銅(Ⅱ)的相互作用。光譜學(xué)與光譜分析,2000,20(3):308-310
46. 大連蛇島蝮蛇類(lèi)凝血酶基因克隆與表達(dá)研究。大連理工大學(xué)學(xué)報(bào),2003,43(2):156-159
47. 新的重組蛋白表達(dá)系統(tǒng)——植物根分泌和葉分泌。生物化學(xué)與生物物理進(jìn)展,2003,30(1):128
48. 抗乙肝病毒表面抗原PreS1(20-47)的單鏈抗體在轉(zhuǎn)基因煙草根分泌物中的初步表達(dá)。高技術(shù)通訊,2003,13(9):35-38
49. Variation and characterization analysis of partial fragment of fibroin gene from silkworm,Antheraea pernyi. High Technology Letters,2003,9(3):29-32
50. 喜樹(shù)堿生物合成途徑及其代謝調(diào)控的研究進(jìn)展。高技術(shù)通訊,2004,14(5):106-110
51. 菟絲子提取物對(duì)PC12細(xì)胞中GAP-43表達(dá)影響。大連理工大學(xué)學(xué)報(bào),2004,44(3):378-382
52. Rapid Amplification of 5u2032cDNA End of Suaeda liaotungensis Choline Monooxygenase Using Inverse PCR RACE. High Technol Lett 2002,8(1):5-7
53. Separation and purification of betaine aldehyde dehydrogenase from wild Suaeda liaotungensis. High Technology Letters,2002,8(2):5-17
54. Cloning a Full-length cDNA Encoding UDP-glucose Pyrophosphorylase from Amorpha Fruticosa by PCR-Based Methods. High Technology Letters,2002,8(3):5-10
55. An Lijia. A co-expression system based on phage and phagemid to select cognate antibody-antigen pairs in vivo. High Technology Letters,2002,8(2):5-10
56. Construction of Large Human Single-chain Antibody Phage Display Library. High Technology Letters,2002,8(3):1-4
57. 精子干細(xì)胞轉(zhuǎn)染法制備轉(zhuǎn)基因兔的研究。高技術(shù)通訊,2001,11(130):17-21
58. 反向嵌套PCR高效擴(kuò)增遼寧堿蓬甜菜堿醛脫氫酶cDNA 5u2019末端序列。高技術(shù)通訊,2001,1(11):17-19
59. Research on telomerase detection of activity by combining DNA sequence analysis with TRAP. High Technology Letters,2001,7(3):8-10
60. 新型染料配基對(duì)堿性磷酸酶的親合純化。高;瘜W(xué)工程學(xué)報(bào),2001,5(6):563-567
61. Separation and Purification of Thrombin-like Enzyme by Affinity Adsorbents. High Technology Letters,2001,7(4):13-15
62. 卵黃免疫球蛋白配基親和純化堿性磷酸酶。中國(guó)農(nóng)業(yè)科學(xué),2004,37(6):908-911
相關(guān)圖書(shū)
英漢生物技術(shù)詞匯
作者:安利佳等 ISBN:750253978
出版社:化學(xué)工業(yè)出版社 出版日期:2003-01-01
科研成果
共承擔(dān)國(guó)家、省、市課題10項(xiàng),項(xiàng)目經(jīng)費(fèi)總計(jì)900萬(wàn)元;
申報(bào)國(guó)內(nèi)外專利13項(xiàng),其中國(guó)際專利1項(xiàng);
由安利佳教授為負(fù)責(zé)人完成的“一種植物分子育種新技術(shù)的建立與應(yīng)用”項(xiàng)目鑒定會(huì),于2004年12月19日通過(guò)了遼寧省科技廳科技成果鑒定,達(dá)到了國(guó)際領(lǐng)先水平。
所主持的遼寧省科技廳項(xiàng)目“基因工程關(guān)鍵技術(shù)”和大連市科技局項(xiàng)目“無(wú)載體、無(wú)標(biāo)記基因植物轉(zhuǎn)基因技術(shù)”于2004年4月6日通過(guò)了遼寧省科技廳科技成果驗(yàn)收和大連市科技局科技成果鑒定,達(dá)到了國(guó)際先進(jìn)水平。
獲得榮譽(yù)
2004年安利佳教授率領(lǐng)的課題組被學(xué)校評(píng)為大連理工大學(xué)科研創(chuàng)新團(tuán)隊(duì)。