研究及工作經(jīng)歷
2009年4月——2014年8月東京大學(xué)、日本筑波大學(xué)博士后研究工作
2011年4月——2013年3月日本學(xué)術(shù)振興會(JSPS)特別研究員
研究內(nèi)容
研究領(lǐng)域為環(huán)境微生物、病原微生物及工業(yè)微生物的分子生物學(xué)。探索微生物環(huán)境脅迫、信號傳導(dǎo)及次級代謝調(diào)控等相關(guān)的諸多功能基因，活用這些基因使之服務(wù)于工業(yè)，農(nóng)業(yè)，醫(yī)療衛(wèi)生等領(lǐng)域。長期以來以模型真菌為研究對象，探索了天然免疫殺菌分子一氧化氮（NO）的殺菌機制及病原菌應(yīng)對NO的抗逆機制，鑒定出一系列新型NO抗性功能基因，并闡明了其抗性原理，為新型抗真菌藥物開發(fā)提供了理論基礎(chǔ)。近期又開展了微生物氧化應(yīng)激及與之對應(yīng)的信號傳導(dǎo)、細(xì)胞凋亡與衰老等相關(guān)機制研究。
研究方向
1.真菌的NO抗性分子機制解析及抗真菌藥物殺菌靶點的定位
2.真菌的氧化應(yīng)激抗性體系的解析及新型殺菌靶點的探索
3.真菌有用次級代謝產(chǎn)物合成途徑及其調(diào)控機制的研究
4.工業(yè)微生物生物被膜形成的分子機制及其集群效應(yīng)
5.智能型絲狀真菌蛋白表達(dá)體系的開發(fā)
6.活體檢測生物探針的開發(fā)
代表論文
(1).Yao Zhou,Shengmin Zhou*, Haijun Yu, Jingyi Li, Yang Xia, Baoyi Li, Xiaoli Wang, Ping Wang*. Cloning and characterization of filamentous fungal S-nitrosoglutathione reductase from Aspergillus nidulans.Journal of Microbiology and Biotechnology. In press, doi: 10.4014/jmb.1512.12009, Feb, 2016
(2).Jia Wei, Xiaodan Cao,Shengmin Zhou, Chao Chen, Haijun Yu, Yao Zhou, Ping Wang. Soluble expression and purification of the catalytic domain of human vascular endothelial growth factor receptor 2 in Escherichia coli.Journal of Microbiology and Biotechnology. 25(8): 1227-1233, 2015
(3).Shunsuke Masuo, Lisa Osada,Shengmin Zhou, Tomoya Fujita, Naoki Takaya. Aspergillus oryzae pathways that convert phenylalanine into the flavor volatile 2-phenylethanol.Fungal Genetics and Biology. 77: 22-30, 2015.
(4).Shengmin Zhouand Naoki Takaya. Novel fungal nitrosothionein peptide involved in tolerance to nitric oxide.Bioscience&industry. 72(2): 55-56, 2014.
(5).Masanori Toyofuku,Shengmin Zhou, Isao Sawada, Naoki Takaya, Hiroo Uchiyama and Nobuhiko Nomura. Membrane vesicle formation is associated with pyocin production under denitrifying conditions in Pseudomonas aeruginosa PAO1.Environmental Microbiology.16(9):2927-2938, Sep, 2014,
(6).Shengmin Zhou, Toshiaki Narukami, ShunsukeMasuo, Motoyuki Shimizu, Tomoya Fujita, Yuki Doi, Yosuke Kamimura, Naoki Takaya. NO-inducible nitrosothionein mediates NO removal in tandem with thioredoxin.Nature Chemical Biology. 9(10): 657-663, Aug, 2013.
(7).Tomoya Fujita, HieuDuc Nguyen, Takashi Ito,Shengmin Zhou, Lisa Osada, Seiji Tateyama, Tatsuo Kaneko, Naoki Takaya. Microbial monomers custom-synthesized to build true bio-derived aromatic polymers.Applied Microbiology and Biotechnology. 97(20):8887-8894, Aug, 2013.
(8).Wenjing Cui, Xiaoyan Yang, Yueqin Fang,Shengmin Zhou, Song Liu, Guocheng Du, Kun Du, Jian Chen, Guanjun Tao and Zhemin Zhou. Discovery of two transglutaminases derived from same zymogen for the Streptomyces hygroscopicus and analysis of their formation processes.Journal of the Science of Food and Agriculture. 93(7): 1711-1717, May, 2013.
(9).Morio Miyahara, Sang-Wan Kim,Shengmin Zhou, Shinya Fushinobu, Takeshi Yamada, Wakako Ikeda-Ohtsubo, Akira Watanabe, Keisuke Miyauchi, Ginro Endo and Hirofumi Shoun. Survival of the aerobic denitrifier Pseudomonas stutzeri strain TR2 during co-culture with activated sludge under denitrifying conditions.Bioscience, Biotechnology, and Biochemistry. 76(3): 495-500, Mar, 2012.
(10).Shengmin Zhou, Toshiaki Narukami, Misuzu Nameki, Tomoko Ozawa, Yosuke Kamimura, Takayuki Hoshino and Naoki Takaya. Heme-biosynthetic porphobilinogen deaminase protects Aspergillus nidulans from nitrosative stress.Applied and Environmental Microbiology. 78(1): 103-109, Jan, 2012.
(11).Shengmin Zhou, Shinya Fushinobu, Sang-Wan Kim, Yoshito Nakanishi, Jun-ichi Maruyama, Katsuhiko Kitamoto, Takayoshi Wakagi, and Hirofumi Shoun. Functional analysis and subcellular location of two flavohemoglobins from Aspergillus oryzae.Fungal Genetics and Biology.48(2): 200-207, Feb, 2011.
(12).Sang-Wan Kim, Shinya Fushinobu,Shengmin Zhou, Takayoshi Wakagi and Hirofumi Shoun. The possible involvement of copper-containing nitrite reductase (NirK) and flavohemoglobin in denitrification by the fungus Cylindrocarpon tonkinense.Bioscience, Biotechnology, and Biochemistry.74 (7): 1403-1407, Jul, 2010.
(13).Shengmin Zhou*, Yoshito Nakanishi*, Shinya Fushinobu, Sang-Wan Kim, Jun-ichi Maruyama, Katsuhiko Kitamoto, Takayoshi Wakagi, and Hirofumi Shoun. A eukaryotic copper-containing nitrite reductase derived from a NirK homolog gene of Aspergillus oryzae.Bioscience, Biotechnology, and Biochemistry.74(5):984-991, May, 2010.
(14).Shengmin Zhou, Shinya Fushinobu, Sang-Wan Kim, Yoshito Nakanishi, TakayoshiWakagi, and Hirofumi Shoun. Aspergillus oryzae flavohemoglobins promote oxidative damage by hydrogen peroxide.Biochemical and Biophysical Research Communications.394(3):558-561, Apr, 2010.
(15).Sang-Wan Kim, Shinya Fushinobu,Shengmin Zhou, Takayoshi Wakagi, and Hirofumi Shoun. Eukaryotic nirK genes encoding copper-containing nitrite reductase: originating from the protomitochondrion?Applied and Environmental Microbiology. 75 (9): 2652-2658, Mar, 2009.
(16).Shengmin Zhou, Shinya Fushinobu, Yoshito Nakanishi, Sang-Wan Kim, Takayoshi Wakagi and Hirofumi Shoun. Cloning and characterization of two flavohemoglobins from Aspergillus oryzae.Biochemical and Biophysical Research Communications.381 (1): 7-11, Jan, 2009.
