1. 新型基于光-電的生物傳感器的構(gòu)建及其在表觀遺傳修飾檢測(cè)方面的應(yīng)用研究（如microRNA、DNA甲基化、DNA羥甲基化、組蛋白磷酸化、蛋白激酶、組蛋白乙�；�、組蛋白乙酰轉(zhuǎn)移酶等）。

2. 電化學(xué)傳感器在食品分析中的應(yīng)用研究。

3. 電化學(xué)傳感器在酚類環(huán)境污染物檢測(cè)中的應(yīng)用研究。

4. 土壤重金屬污染的修復(fù)及機(jī)理研究。

1. 國(guó)家自然科學(xué)基金——面上項(xiàng)目，2018.1-2021.12,64萬元。

2. 國(guó)家自然科學(xué)基金——青年項(xiàng)目，2012.1-2014.12，25萬元。

3. 中國(guó)博士后基金一等資助，2017.7，8萬元。

4. 中國(guó)博士后科學(xué)基金特別資助，2015.7，15萬元。

5. 山東省自然科學(xué)基金——青年項(xiàng)目，2011.7-2014.7，6萬元。

6. 山東省自然科學(xué)基金——面上項(xiàng)目，2017.1-2019.12，17萬元。

7. 作物生物學(xué)國(guó)家重點(diǎn)實(shí)驗(yàn)室開放課題，2014.1-2016.12，4萬元。

8. 山東農(nóng)業(yè)大學(xué)杰出青年基金1項(xiàng)，2017.1-2020.12，60萬元。

9. 山東農(nóng)業(yè)大學(xué)博士后項(xiàng)目1項(xiàng)，4萬元。

2018年

[1]Haiyan Wang, Chunling Qi, Wenhuan He, Minghui Wang, Wenjing Jiang, Huanshun Yin*, Shiyun Ai*, A sensitive photoelectrochemical immunoassay of N6-methyladenosine based on dual-signal amplification strategy: Ru doped in SiO2 nanosphere and carboxylated g-C3N4, Biosensors and Bioelectronics, 2018, 99, 281-288.

[2]Wenjing Jiang, Linni Wu, JunlingDuan, Huanshun Yin*, Shiyun Ai, Ultrasensitive electrochemiluminescence immunosensor for 5-hydroxymethylcytosine detection based on Fe3O4@SiO2 nanoparticles and PAMAM dendrimers, Biosensors and Bioelectronics, 2018, 99, 660-666

2017年

[1]Yunlei Zhou, Minghui Wang, Huanshun Yin*, Shiyun Ai, Amperometric determination of the activity of protein kinase a using a glassy carbon electrode modified with IgG functionalized gold nanoparticles conjugated to horseradish peroxidase, Microchimica Acta, 2017, 184, 3301-3308.

[2]Huanshun Yin, Zhiqing Yang, Haiyan Wang, Yunlei Zhou*, Shiyun Ai, Electrochemical biosensor for hydroxymethylated DNA detection and β-glucosyltransferase activity assay based on enzymatic catalysis triggering signal amplification, Sensors and Actuators B: Chemical, 2017, 243, 602-608.

[3]Huanshun Yin, Haiyan Wang, Wenjing Jiang, Yunlei Zhou*, Shiyun Ai*, Electrochemical immunosensor for N6-methyladenosine detection in human cell lines based on biotin-streptavidin system and silver-SiO2?signal amplification, Biosensors and Bioelectronics, 2017, 90, 494-500.

[4]Haiyan Wang, Qihai Zhang, Huanshun Yin*, Minghui Wang, Wenjing Jiang, Shiyun Ai*, Photoelectrochemicalimmunosensor for methylated RNA detection based on g-C3N4/CdS quantum dots heterojunction and Phos-tag-biotin, Biosensors and Bioelectronics, 2017, 95, 124-130.

[5]Haiyan Wang, Pei Liu, Wenjing Jiang, Xue Li, Huanshun Yin*, Shiyun Ai, Photoelectrochemical immunosensing platform for M. SssI methyltransferase activity analysis and inhibitor screening based on g-C3N4?and CdS quantum dots, Sensors and Actuators B: Chemical, 2017, 244, 458-465.

[6]Xue Li, Lusheng Zhu, Yunlei Zhou, Huanshun Yin*, Shiyun Ai*, Enhanced photoelectrochemical method for sensitive detection of protein kinase A activity using TiO2/g-C3N4, PAMAM dendrimer, and alkaline phosphatase, Analytical Chemistry, 2017, 89, 2369-2376.

2016年

[1]Yunlei Zhou, Huanshun Yin*, Xue Li, Zhi Li, Shiyun Ai, Hai Lin*, Electrochemical biosensor for protein kinase A activity assay based on gold nanoparticles-carbon nanospheres, phos-tag-biotin and β-galactosidase, Biosensors and Bioelectronics, 2016, 86, 508-515.

[2]Yunlei Zhou, Huanshun Yin*, Jie Li, Bingchen Li, Xue Li, Shiyun Ai, Xiansheng Zhang*, Electrochemical biosensor for microRNA detection based on poly(U) polymerase mediated isothermal signal amplification, Biosensors and Bioelectronics, 2016, 79, 79-85.

[3]Yunlei Zhou, Mo Wang, Zhiqing Yang, Linan Lu, Huanshun Yin*, Shiyun Ai*, Electrochemical biosensor for microRNA detection based on hybridization protection against nuclease S1 digestion, Journal of Solid State Electrochemistry, 2016, 20, 413-419.

[4]Qingming Zhang, Zhi Li, Yunlei Zhou, Xue Li, Bingchen Li, Huanshun Yin*, Shiyun Ai, Electrochemical biosensors for polynucleotide kinase activity assay and inhibition screening based on phosphorylation reaction triggered λ exonuclease and exonuclease I cleavage, Sensors and Actuators B: Chemical, 2016, 225, 151-157.

[5]Huanshun Yin, Yunlei Zhou, Bingchen Li, Xue Li, Zhiqing Yang, Shiyun Ai*, Xiansheng Zhang*, Photoelectrochemical immunosensor for microRNA detection based on gold nanoparticles-functionalized g-C3N4?and anti-DNA:RNA antibody, Sensors and Actuators B: Chemical, 2016, 222, 1119-1126.

[6]Zhiqing Yang, Yanhua Shi, Wenrong Liao, Huanshun Yin*, Shiyun Ai*, A novel signal-on photoelectrochemical biosensor for detection of 5-hydroxymethylcytosine based on in situ electron donor producing strategy and all wavelengths of light irradiation, Sensors and Actuators B: Chemical, 2016, 223, 621-625.

[7]Pei Liu, Dandan Wang, Yunlei Zhou, Haiyan Wang, Huanshun Yin*, Shiyun Ai*, DNA methyltransferase detection based on digestion triggering the combination of poly adenine DNA with gold nanoparticles, Biosensors and Bioelectronics, 2016, 80, 74-78.

[8]Xue Li, Yunlei Zhou, Yan Xu, HuijieXu, Minghui Wang, Huanshun Yin*, Shiyun Ai*, A novel photoelectrochemical biosensor for protein kinase activity assay based on phosphorylated graphite-like carbon nitride, Analytica Chimica Acta, 2016, 934, 36-43.

[9]Bingchen Li, Fei Liu, YuanyuanPeng, Yunlei Zhou, Wenxuan Fan, Huanshun Yin*, ShiyunAi*, Xiansheng Zhang*, Two-stage cyclic enzymatic amplification method for ultrasensitive electrochemical assay of microRNA-21 in the blood serum of gastric cancer patients, Biosensors and Bioelectronics, 2016, 79, 307-312.

2015年

[1]Yunlei Zhou, Zhiqing Yang, Xue Li, Yue Wang, Huanshun Yin*, Shiyun Ai*, Electrochemical biosensor for detection of DNA hydroxymethylation based on glycosylation and alkaline phosphatase catalytic signal amplification, Electrochimica Acta, 2015, 174, 647-652.

[2]Yunlei Zhou, Mo Wang, Zhiqing Yang, Huanshun Yin*, Shiyun Ai*, A Phos-tag-based photoelectrochemical biosensor for assay of protein kinase activity and inhibitors, Sensors and Actuators B: Chemical, 2015, 206, 728-734.

[3]Qingming Zhang, Xue Li, Bingchen Li, Huanshun Yin*, Shiyun Ai, An electrochemical biosensor for the activity assay of polynucleotide kinase and inhibitor screening, Analytical Methods, 2015, 7, 9984-9991.

[4]Huanshun Yinu2020, Yunlei Zhouu2020, Zhiqing Yang, YunlongGuo, Xinxu Wang, Shiyun Ai*, Xiansheng Zhang*, Electrochemical immunosensor for N6-methyladenosine RNA modification detection, Sensors and Actuators B: Chemical, 2015, 221, 1-6.

[5]Huanshun Yin, Zhiqing Yang, Bingchen Li, Yunlei Zhou*, Shiyun Ai*, Electrochemical biosensor for DNA demethylase detection based on demethylation triggered endonuclease BstUI and Exonuclease III digestion, Biosensors and Bioelectronics, 2015, 66, 266-270.

[6]Huanshun Yin, Xinxu Wang, YunlongGuo, Yunlei Zhou*, Shiyun Ai*, Electrochemical detection of protein kinase activity based on carboxypeptidase Y digestion triggered signal amplification, Biosensors and Bioelectronics, 2015, 66, 77-83.

[7]Huanshun Yin, Mo Wang, Bingchen Li, Zhiqing Yang, Yunlei Zhou*, Shiyun Ai*, A sensitive electrochemical biosensor for detection of protein kinase A activity and inhibitors based on Phos-tag and enzymatic signal amplification, Biosensors and Bioelectronics, 2015, 63, 26-32.

[8]Huanshun Yin, Bing Sun, Linfeng Dong, Bingchen Li, Yunlei Zhou*, Shiyun Ai, * A signal-on photoelectrochemical biosensor for assay of protein kinase activity and its inhibitor based on graphite-like carbon nitride, Phos-tag and alkaline phosphatase, Biosensors and Bioelectronics, 2015, 64, 462-468.

[9]Zhiqing Yang, Fengrui Wang, Mo Wang, Huanshun Yin*, Shiyun Ai*, A novel signal-on strategy for M.SssI methyltransfease activity analysis and inhibitor screening based on photoelectrochemical immunosensor, Biosensors and Bioelectronics, 2015, 66, 109-114.

[10]Zhiqing Yang, Wenjing Jiang, Fei Liu, Yunlei Zhou, Huanshun Yin*, Shiyun Ai*, A novel electrochemical immunosensor for the quantitative detection of 5-hydroxymethylcytosine in genomic DNA of breast cancer tissue, Chemical Communications, 2015, 51, 14671-14673.

[11]Mo Wang, Zhiqing Yang, YunlongGuo, Xinxu Wang, Huanshun Yin*, Shiyun Ai*, Visible-light induced photoelectrochemical biosensor for the detection of microRNA based on Bi2S3 nanorods and streptavidin on an ITO electrode, Microchimica Acta, 2015, 182, 241-248.

[12]Pei Liu, Junling Pang, Huanshun Yin*, Shiyun Ai*, G-quadruplex functionalized nanomesoporous silica for assay of the DNA methyltransferase activity, Analytica Chimica Acta, 2015, 879, 34-40.

[13]Bingchen Li, Xue Li, Mo Wang, Zhiqing Yang, Huanshun Yin*, Shiyun Ai*, Photoelectrochemical biosensor for highly sensitive detection of microRNA based on duplex-specific nuclease-triggered signal amplification, Journal of Solid State Electrochemistry, 2015, 19, 1301-1309.

2014年

[1]Yunlei Zhou, ZhenningXu, Mo Wang, Bing Sun, Huanshun Yin*, Shiyun Ai*, DNA methyltransferase activity assay based on visible light-activated photoelectrochemical biosensor, Biosensors and Bioelectronics, 2014, 53, 263-267.

[2]Yunlei Zhou, Mo Wang, ZhenningXu, Cailing Ni, Huanshun Yin*, Shiyun Ai*, Investigation of the effect of phytohormone on the expression of microRNA-159a in Arabidopsis thaliana seedlings based on mimic enzyme catalysis systematic electrochemical biosensor, Biosensors and Bioelectronics, 2014, 54, 244-250.

[3]Yunlei Zhou, Bingchen Li, Mo Wang, Zhiqing Yang, Huanshun Yin*, Shiyun Ai*, Enzyme-based electrochemical biosensor for sensitive detection of DNA demethylation and the activity of DNA demethylase, Analytica Chimica Acta, 2014, 840, 28-32.

[4]Huanshun Yin, Mo Wang, Yunlei Zhou, Xiaoyan Zhang, Bing Sun, Guihua Wang, Shiyun Ai*, Photoelectrochemical biosensing platform for microRNA detection based on in situ?producing electron donor from apoferritin-encapsulated ascorbic acid, Biosensors and Bioelectronics, 2014, 53, 175-181.

[5]ZhenningXu, Huanshun Yin, Yunxiang Han, Yunlei Zhou *, Shiyun Ai *, DNA-based hybridization chain reaction amplification for assaying the effect of environmental phenolic hormone on DNA methyltransferase activity, Analytica Chimica Acta, 2014, 829, 9u201314.

[6]Huanshun Yin, Bing Sun, Yunlei Zhou, Mo Wang, ZhenningXu, Zhengliang Fu, Shiyun Ai*, A new strategy for methylated DNA detection based on photoelectrochemical immunosensor using Bi2S3nanorods, methyl bonding domain protein and anti-his tag antibody, Biosensors and Bioelectronics, 2014, 51, 103-108.

[7]ZhenningXu, Huanshun Yin*, Yunxiang Han, Yunlei Zhou, Shiyun Ai*, DNA-based hybridization chain reaction amplification for assaying the effect of environmental phenolic hormone on DNA methyltransferase activity, Analytica Chimica Acta, 2014, 829, 9-14.

[8]Mo Wang, Huanshun Yin*, NannanShen, ZhenningXu, Bing Sun, Shiyun Ai*, Signal-on photoelectrochemical biosensor for micro RNA detection based on Bi2S3 nanorods and enzymatic amplification, Biosensors and Bioelectronics, 2014, 53, 232-237.

[9]Mo Wang, Zhengliang Fu, Bingchen Li, Yunlei Zhou, Huanshun Yin*, Shiyun Ai*, One-step, ultrasensitive and electrochemical assay of microRNAs based on T7 exonuclease assisted cyclic enzymatic amplification, Analytical Chemistry, 2014, 86, 5606-5610.

[10]Bing Sun, Lijian Chen, Yan Xu, Min Liu, Huanshun Yin*, Shiyun Ai*, Ultrasensitive photoelectrochemical immunoassay of indole-3-acetic acid based on the MPA modified CdS/RGO nanocomposites decorated ITO electrode, Biosensors and Bioelectronics, 2014, 51, 164-169.

2013年

[1]Yunlei Zhou, ZhenningXu, Mo Wang, XiaomengMeng, Huanshun Yin*, Electrochemical immunoassay platform for high sensitivity detection of indole-3-acetic acid, Electrochimica Acta, 2013, 96, 66-73.

[2]Huanshun Yinu2020, Yunlei Zhouu2020, ZhenningXu, Mo Wang, Shiyun Ai*, Ultrasensitive electrochemical immunoassay for DNA methyltransferase activity and inhibitor screening based on methyl binding domain protein of MeCP2 and enzymatic signal amplification, Biosensors and Bioelectronics, 2013, 49, 39-45.

[3]Huanshun Yinu2020, Yunlei Zhouu2020, ZhenningXu, Lijian Chen, Di Zhang, Shiyun Ai*, An electrochemical assay for DNA methylation, methyltransferase activity and inhibitor screening based on methyl binding domain protein, Biosensors and Bioelectronics, 2013, 41, 492-497.

[4]Huanshun Yin, ZhenningXu, Yunlei Zhou, Mo Wang, Shiyun Ai*, An ultrasensitive electrochemical immunosensor platform with double signal amplification for indole-3-acetic acid determinations in plant seeds, Analyst, 2013, 138, 1851-1857.

[5]ZhenningXu, Mo Wang, Tingting Zhou, Huanshun Yin*, Shiyun Ai*, Electrochemical biosensing method for the detection of DNA methylation and assay of the methyltransferase activity, Sensors and Actuators B: Chemical, 2013, 178, 412-417.

[6]ZhenningXu, Mo Wang, Huanshun Yin*, Shiyun Ai*, Liang Wang, Junling Pang, A sensitive electrochemical method for DNA methyltransferase assay and inhibitor screening based on DNA methylation-sensitive cleavage, ElectrochimicaActa, 2013, 112, 596-602.

[7]XiaomengMengu2020, Yunlei Zhouu2020, Qianjin Liang, XiangjinQu, Qingqing Yang, Huanshun Yin*, Shiyun Ai*, Electrochemical determination of microRNA-21 based on bio bar code and hemin/G-quadruplet DNAenzyme, Analyst, 2013, 138, 3409-3415.

[8]XiaomengMeng, ZhenningXu, Mo Wang, Huanshun Yin*, Shiyun Ai*, Direct determination of 5-methylcytosine based on electrochemical activation of surfactant functionalized graphene modified pyrolytic graphite electrode, Electrochimica Acta, 2013, 95, 200-204.

[9]Huanshun Yin, ZhenningXu, Mo Wang, Xueping Zhang, Shiyun Ai*, An electrochemical biosensor for assay of DNA methyltransferase activity and screening of inhibitor, Electrochimica Acta, 2013, 89, 530-536.

2012年

[1]Yunlei Zhou, Zhaoyan Zhang, ZhenningXu, Huanshun Yin*, Shiyun Ai*, MicroRNA-21 detection based on molecular switching by amperometry, New Journal of Chemistry, 2012, 36, 1985-1991.

[2]Yunlei Zhou, Huanshun Yin*, XiaomengMeng, ZhenningXu, Yanrong Fu, Shiyun Ai, Direct electrochemistry of sarcosine oxidase on graphene, chitosan and silver nanoparticles modified glassy carbon electrode and its biosensing for hydrogen peroxide, Electrochimica Acta, 2012, 71, 294-301.

[3]Yunlei Zhou, Mo Wang, XiaomengMeng, Huanshun Yin*, Shiyun Ai*, Amplified electrochemical microRNA biosensor using a hemin-G-quadruplex complex as the sensing element, RSC Advances, 2012, 2, 7140-7145.

[4]Huanshun Yinu2020, Yunlei Zhouu2020, Haixia Zhang, XiaomengMeng, Shiyun Ai*, Electrochemical determination of microRNA-21 based on graphene, LNA integrated molecular beacon, AuNPs and biotin multifunctional bio bar codes and enzymatic assay system, Biosensors and Bioelectronics, 2012, 33, 247-253.

[5]Huanshun Yin, Yunlei Zhou, Tao Liu, Tiantian Tang, Shiyun Ai*, Lusheng Zhu*, Determination aminopyrine in pharmaceutical formulations based on APTS-Fe3O4?nanoparticles modified glassy carbon electrode, Journal of Solid State Electrochemistry, 2012, 16, 731-738.

[6]Huanshun Yin, Yunlei Zhou, Ruixia Han, YanyanQiu, Shiyun Ai*, Lusheng Zhu*, Electrochemical oxidation behavior of 2,4-dinitrophenol at hydroxylapatite film-modified glassy carbon electrode and its determination in water samples, Journal of Solid State Electrochemistry, 2012, 16, 75-82.

[7]Huanshun Yin, Yunlei Zhou, Chuanxia Chen, Lusheng Zhu*, Shiyun Ai*, An electrochemical signal ’off-on’ sensing platform for micro RNA detection, Analyst, 2012, 137, 1389-1395.

[8]Huanshun Yin, Yunlei Zhou, Shiyun Ai*, Qiang Ma, Lushengzhu*, Linan Lu, Electrochemical oxidation determination and voltammetricbehaviour of 4-nitrophenol based on Cu2O nanoparticles modified glassy carbon electrode, International Journal of Environmental Analytical Chemistry, 2012, 92, 742-754.

[9]Huanshun Yin, XiaomengMeng, ZhenningXu, Lijian Chen, Shiyun Ai*, Electrochemical behavior of phenacetin on CdSe microspheres modified glassy carbon electrode and its simultaneous determination with paracetamol and 4-aminophenol, Analytical Methods, 2012, 4, 1445-1451.

[10]Huanshun Yin, XiaomengMeng, Haichao Su, MinrongXu, Shiyun Ai*, Electrochemical determination of theophylline in foodstuff, tea and soft drinks based on urchin-like CdSemicroparticles modified glassy carbon electrode, Food Chemistry, 2012, 134, 1225-1230.

[11]Mo Wang, ZhenningXu, Lijian Chen, Huanshun Yin*, Shiyun Ai*, An electrochemical immunosensing platform for DNA methyltransferase activity analysis and inhibitor screening, Analytical Chemistry, 2012, 84, 9072-9078.

[12]XiaomengMeng, ZhenningXu, Mo Wang, Huanshun Yin*, Shiyun Ai, Electrochemical behavior of antipyrine at a Bi2S3?modified glassy carbon electrode and its determination in pharmaceutical formulations, Analytical Methods, 2012, 4, 1736-1741.

1. 2011年，山東高等學(xué)校優(yōu)秀科研成果獎(jiǎng)—自然科學(xué)類二等獎(jiǎng)，排名第二位。

2. 2011年，山東農(nóng)業(yè)大學(xué)“突出貢獻(xiàn)獎(jiǎng)先進(jìn)個(gè)人”。

3. 2012年，指導(dǎo)2007級(jí)應(yīng)用化學(xué)本科專業(yè)張海霞的“基于信號(hào)擴(kuò)增的生物傳感器檢測(cè)MircoRNA-21”論文，被評(píng)為“2012年山東省優(yōu)秀學(xué)士學(xué)位論文”。

4. 2012年，山東農(nóng)業(yè)大學(xué)“突出貢獻(xiàn)獎(jiǎng)先進(jìn)個(gè)人”。

5. 2013年，山東農(nóng)業(yè)大學(xué)“青年崗位能手標(biāo)兵”。

6. 2013年，山東高等學(xué)校優(yōu)秀科研成果獎(jiǎng)—自然科學(xué)類二等獎(jiǎng)，排名第二位。

7. 2013年，?“第十一屆泰安市青年科技獎(jiǎng)”。

8. 2013年，指導(dǎo)2008級(jí)應(yīng)用化學(xué)本科專業(yè)王默的本科畢業(yè)論文“電化學(xué)免疫傳感器檢測(cè)DNA甲基化及甲基酶活性”論文被評(píng)為“2013年山東省優(yōu)秀學(xué)士學(xué)位論文”。

9. 2015年，山東高等學(xué)校優(yōu)秀科研成果獎(jiǎng)—自然科學(xué)類三等獎(jiǎng)，排名第一位。

10. 2015年，山東高等學(xué)校優(yōu)秀科研成果獎(jiǎng)—自然科學(xué)類三等獎(jiǎng)，排名第二位。

11.2016年，山東高等學(xué)校優(yōu)秀科研成果獎(jiǎng)—自然科學(xué)類二等獎(jiǎng)，排名第一位。

12. 2016年，山東省自然科學(xué)獎(jiǎng)三等獎(jiǎng)，排名第二位。