馮耀宇，女，1969年出生，現(xiàn)任同濟大學(xué)生命科學(xué)與技術(shù)學(xué)院教授，博士生導(dǎo)師；以及同濟大學(xué)海洋與地球科學(xué)學(xué)院兼職博士生導(dǎo)師。

　　于1990年和1993年分別獲得南開大學(xué)微生物專業(yè)的本科和碩士學(xué)位，隨后進入天津大學(xué)從事生物化工領(lǐng)域的教學(xué)與科研工作，曾參加天津市導(dǎo)向性重大科技項目和多項國家自然科學(xué)基金等項目的研究，并于1999年獲得生物化工專業(yè)的博士學(xué)位。2000年初至2004年6月在新加坡國立大學(xué)土木工程系水研究中心從事博士后研究員工作，參加多項新加坡政府和新加坡國立大學(xué)研究項目, 主要從事環(huán)境微生物，尤其是水中新發(fā)寄生蟲的檢測工作，并取得國際領(lǐng)先的研究成果。研究對象廣泛涉及微生物多個領(lǐng)域，主要包括細菌，病毒和原生動物等。研究目的包括新發(fā)現(xiàn)的水生病原菌的檢測方法建立及應(yīng)用多種水處理工藝去除這些病原菌。最新研究方向還包括雌性激素類化合物的生物檢測方法建立及它們在水處理工藝中的去除研究。2004年6月至今作為教授、博導(dǎo)在同濟大學(xué)工作期間，繼續(xù)從事寄生蟲病檢測方面的研究。2005年9月至今在美國疾病預(yù)防控制中心作為國際新發(fā)傳染病研究員，主要從事多種寄生蟲病的分子流行病學(xué)研究。

　　近年來共主持國內(nèi)項目2項, 國外項目3項，包括國際合作項目等，獲科研基金總額近兩百萬元。在水體中細菌，病毒和原生動物檢測和去除方面進行了長期研究，尤其在新發(fā)現(xiàn)的水體中寄生蟲類病原菌的檢測方法建立方面取得了驕人的成績。研究成果修訂了美國國家環(huán)保局的相應(yīng)檢測方法標準；并首次報道了運用新型納米熒光材料對檢測寄生蟲的感染性進行免疫熒光標定的技術(shù)，從而建立了一種國際領(lǐng)先的可以同時測定多種對人類致病的隱孢子蟲感染性的新方法；同時還報道了一種可以同時檢測多種對人類致病的寄生蟲的感染性、定量及種類區(qū)分的國際領(lǐng)先新型技術(shù)。此外，還首次構(gòu)建了一種新型的可利用淀粉高產(chǎn)堿性蛋白酶的工程菌，并對酶的性質(zhì)、純化和生產(chǎn)工藝等進行了詳盡研究，同時篩選出了甘露聚糖酶的高產(chǎn)菌株及對其進行了詳盡的不同規(guī)模的生產(chǎn)和純化工藝及產(chǎn)酶動力學(xué)研究，該工作使得這兩種酶成功進行了技術(shù)轉(zhuǎn)讓。

　　主要成績： 1.研究獎項：榮獲2001年天津市自然科學(xué)一等獎。2. 期刊論文：國外重要學(xué)術(shù)刊物發(fā)表論文32篇（25余篇被SCI收錄），其中某些期刊的SCI影響因子達6以上。3. 會議報告和論文：在國際學(xué)術(shù)會議上做特邀報告2次，發(fā)表國際會議論文10余篇，國內(nèi)會議論文5篇。4. 期刊審稿：擔任“Water Research”，“Biotechnology and Applied Biochemistry”和“Process Biochemistry”等國外重要學(xué)術(shù)刊物的審稿人。5.國際會議審稿：曾擔任5個國際會議的特約審稿人。

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[英文]    Improvement of recoveries for the determination of protozoa Cryptosporidium and Giardia in water using method 1623

Author(s)：Jiangyong Hu*, Yaoyu Feng, Say Leong Ong, Wun Jern Ng, Lianfa Song, Xiaolan Tan, Xiaona Chu

Affiliation：Center for Water Research, Environmental Science and Engineering Program, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore

Source：Journal of Microbiological Methods 58(2004)321-325

KeywordsAbstractPDF

Keywords:  Cryptosporidium; Filtration; Giardia; Immunomagnetic separation; Recovery

Abstract:  The U.S. Environmental Protection Agency has developed method 1623 for simultaneous detection of Cryptosporidium oocysts and Giardia cysts in water. Method 1623 includes four major steps: filtration, immunomagnetic separation (IMS), fluorescent antibody (FA) staining and microscopic examination. It was noted that the recovery levels following IMS-FA and FA staining were high, averaging more than 92.0% and 89.0% for C. parvum oocysts and G. lamblia cysts, respectively. In contrast, when the filtration step was incorporated, the recovery level of C. parvum oocysts declined significantly to 18.1% in seeded tap water, while a relatively high recovery level of 77.2% for G. lamblia cysts could still be achieved. Further study indicated that the recovery level of C. parvum oocysts could be enhanced significantly when an appropriate amount of silica particles was added to a water sample. The recovery level of C. parvum oocysts was affected by particle size and concentration. The optimal silica particle size was determined to be within the range of 5-40 Am, and the corresponding optimal silica concentration was 1.42g for 10-l tap water. When both G. lamblia cysts and C. parvum oocysts were spiked into the tap water sample containing the optimum amount of silica particles, the average recovery levels of oocysts and cysts were 82.7% and 75.4%, respectively. The results obtained clearly suggested that addition of an appropriate amount of silica particles could improve the recovery level of C. parvum oocysts significantly and yet there was no noticeable deleterious effect on the recovery level of G. lamblia cysts. Further study indicated that the rotation time in the IMS procedure using the Dynal GC-Combo IMS kit (which was recommended in method 1623) was important for G. lamblia cyst detection. In contrast, the recovery level of C. parvum oocysts was not affected by the rotation time. Furthermore, it was found that the recovery levels of C. parvum oocysts using methods 1622 and 1623 were quite close although different IMS kits were used in the two methods.

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[英文]    Effect of Particles on the Recovery of Cryptosporidium Oocysts from Source Water Samples of Various Turbidities

Author(s)：Yao Yu Feng, Say Leong Ong,*, Jiang Yong Hu, Lian Fa Song, Xiao Lan Tan, and Wun Jern Ng

Affiliation：Center for Water Research, Department of Civil Engineering, National University of Singapore, Singapore 119260, Singapore

Source：APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Apr. 2003, p. 1898-1903

KeywordsAbstractPDF

Keywords:  NULL.

Abstract:  Cryptosporidium parvum can be found in both source and drinking water and has been reported to cause serious waterborne outbreaks which threaten public health safety. The U.S. Environmental Protection Agency has developed method 1622 for detection of Cryptosporidium oocysts present in water. Method 1622 involves four key processing steps: filtration, immunomagnetic separation (IMS), fluorescent-antibody (FA) staining, and microscopic evaluation. The individual performance of each of these four steps was evaluated in this study. We found that the levels of recovery of C. parvum oocysts at the IMS-FA and FA staining stages were high, averaging more than 95%. In contrast, the level of recovery declined significantly, to 14.4%, when the filtration step was incorporated with tap water as a spiking medium. This observation suggested that a significant fraction of C. parvum oocysts was lost during the filtration step. When C. parvum oocysts were spiked into reclaimed water, tap water, microfiltration filtrate, and reservoir water, the highest mean level of recovery of (85.0%±5.2% [mean±standard deviation]) was obtained for the relatively turbid reservoir water. Further studies indicated that it was the suspended particles present in the reservoir water that contributed to the enhanced C. parvum oocyst recovery. The levels of C. parvum oocyst recovery from spiked reservoir water with different turbidities indicated that particle size and concentration could affect oocyst recovery. Similar observations were also made when silica particles of different sizes and masses were added to seeded tap water. The optimal particle size was determined to be in the range from 5 to 40μm, and the corresponding optimal concentration of suspended particles was 1.42g for 10 liters of tap water.

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[英文]    Optimization of agitation, aeration, and temperature conditions for maximum-mannanase production

Author(s)：Yaoyu Fenga,?, Zhimin Heb, Say Leong Onga, Jiangyong Hua, Zhigang Zhangb, Wun Jern Nga

Affiliation：a Department of Civil Engineering, National University of Singapore, Singapore 119260, Singapore; b Institute of Chemical Engineering, Tianjin University, Tianjin 300072, China

Source：Enzyme and Microbial Technology 32(2003)282-289

KeywordsAbstractPDF

Keywords:  βMannanase; Bacillus licheniformis; Aeration rate; Agitation speed; Temperature

Abstract:  The effects of cultivation temperature, aeration rate, and agitation speed on the production of β-mannanase by Bacillus licheniformis NK-27 in a batch fermenter were investigated in this study. Results revealed that temperature was the most significant factor in terms of its effect on β-mannanase production. It influenced β-mannanase production by affecting the other parameters including bacteria growth, pH, dissolved oxygen, total and reducing sugars. Agitation speed and aeration rate could affect dissolved oxygen concentration which in turn affected cell growth and β-mannanase production. A maximum βmannanase activity of 212.0Uml?1 was attained in 36h of cultivation when aeration rate, agitation speed, and temperature were controlled at 0.75vvm, 600rpm, and 30℃, respectively. The maximum β-mannanase activity in the fermenter was close to that obtained from the shake flask fermentation study (198.2Uml-1). However, the duration of fermentation cycle in the fermenter study was shorter than the corresponding duration obtained from the shake flask experiment by 12h.

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[英文]    Synthesis and characterization of [Ga(TPP)H] (TPP?tetraphenylporphyrinato)

Author(s)：Yaoyu Feng*, Say-Leong Ong, Jiangyong Hu, Wun-Jern Ng

Affiliation：Department of Civil Engineering, National University of Singapore, Singapore 119260, Singapore

Source：Inorganic Chemistry Communications 6(2003)466-468