人物簡(jiǎn)介
王賢純,男,1959年4月出生,1977年參加工作,1982年獲學(xué)士學(xué)位,1986年獲碩士學(xué)位,1998年獲博士學(xué)位,F(xiàn)為湖南師范大學(xué)生命科學(xué)學(xué)院二級(jí)教授,博、碩士生導(dǎo)師;湖南省生物化學(xué)與分子生物學(xué)會(huì)理事,中國(guó)生物化學(xué)與分子生物學(xué)會(huì)第三屆蛋白質(zhì)組學(xué)專業(yè)委員會(huì)委員;蛋白質(zhì)化學(xué)與發(fā)育生物學(xué)教育部重點(diǎn)實(shí)驗(yàn)室副主任、第三屆學(xué)術(shù)委員會(huì)委員;湖南省生物化學(xué)與分子生物學(xué)重點(diǎn)實(shí)驗(yàn)室、教育部蛋白質(zhì)化學(xué)與發(fā)育生物學(xué)重點(diǎn)實(shí)驗(yàn)室和省部共建國(guó)家重點(diǎn)實(shí)驗(yàn)室培育基地主要學(xué)術(shù)帶頭人之一。
學(xué)術(shù)成就
長(zhǎng)期從事生物化學(xué)及相關(guān)學(xué)科的教學(xué)與科研工作。主講生物化學(xué)和蛋白質(zhì)技術(shù)等課程,曾獲生命科學(xué)學(xué)院教師教學(xué)競(jìng)賽一等獎(jiǎng)和湖南師范大學(xué)第四屆青年教師課堂教學(xué)藝術(shù)競(jìng)賽一等獎(jiǎng)。一直擔(dān)任生物化學(xué)與分子生物學(xué)系主任,具體負(fù)責(zé)該系的本科教學(xué)及相關(guān)工作, 帶領(lǐng)該系進(jìn)入湖南師范大學(xué)“三育人”先進(jìn)單位行列。主要從事蛋白質(zhì)化學(xué)、蛋白質(zhì)組學(xué)、天然蛋白質(zhì)多肽毒素的分離純化及結(jié)構(gòu)與功能分析等方面的研究。在相關(guān)研究領(lǐng)域開展系統(tǒng)的探索性工作,逐漸形成穩(wěn)定和具有特色的研究方向。多次應(yīng)邀在國(guó)際國(guó)內(nèi)學(xué)術(shù)會(huì)議上做學(xué)術(shù)報(bào)告。曾先后前往美、英、韓國(guó)和越南參加短期學(xué)習(xí)和學(xué)術(shù)交流。主持國(guó)家863課題和科技部重大基礎(chǔ)研究前期研究專項(xiàng)課題各1項(xiàng),國(guó)家自然科學(xué)基金項(xiàng)目課題4項(xiàng),教育部高等學(xué)校博士學(xué)科點(diǎn)專項(xiàng)科研基金課題2項(xiàng),湖南省自然科學(xué)基金重點(diǎn)課題和一般課題等省級(jí)課題4項(xiàng)。以通訊作者或第一作者在J Proteome Res, PloS One, Electrophoresis,生物化學(xué)與生物物理學(xué)報(bào)和中國(guó)生物化學(xué)與分子生物學(xué)報(bào)等國(guó)內(nèi)外重要學(xué)術(shù)刊物上發(fā)表科研和教研論文70余篇,申報(bào)國(guó)家發(fā)明專利2項(xiàng)。指導(dǎo)培養(yǎng)博士、碩士研究生30余名。參編著作4部。作為主要完成人之一獲2004年和2007年湖南省科技進(jìn)步一等獎(jiǎng)。
主要論文
1. Jianjun Li, Hai Yu, Zhigui Duan, Xianchun Wang. A sample preparation method for
micro-scale membrane proteome analysis. Sam Prep. 2013, 28-36
2. Yi-Zhong Yan, Hui Liu, Rong Lv, Sha Huang, Ji-Rong Wang, Tian-Yao Guo, Qiong Zhou and Xian-Chun Wang. Biotin labeling and receptor analysis of huwentoxin-I. Anal. Methods, 2013, 5, 3888-3894
3. Jianjun Li, Yizhong Yan, Jirong Wang, Tianyao Guo, Weijun Hu, Zhigui Duan, Xianchun Wang, and Songping Liang. Puri?cation and Partial Characterization of a Novel Neurotoxic Protein from Eggs of BlackWidow Spiders (Latrodectus tredecimguttatus). J Biochem Mol Toxicol. 2013, 27(7): 337-342
4. Yong Lin, Linju Huo, Zhonghua Liu, Jianglin Li, Yi Liu, Quanze He, Xianchun Wang, Songping Liang.Sodium Laurate, a Novel Protease- and Mass Spectrometry-Compatible Detergent for MassSpectrometry-Based Membrane Proteomics. PLoS One, 2013, 8(3) : e59779
5. Lin Y, Liu H, Liu Z, Liu Y, He Q, Chen P, Wang X, Liang S. Development and evaluation of an entirely solution-based combinative sample preparation method for membrane proteomics.Anal Biochem. 2013, 432 (1): 41-48
6. Li J, Liu H, Duan Z, Cao R, Wang X, Liang S. Protein Compositional Analysis of the Eggs of Black Widow Spider (L. tredecimguttatus): Implications for the Understanding of Egg Toxicity. J Biochem Mol Toxicol. 2012, 26(12):510-515
7. Xianchun Wang, Songping Liang. Sample preparation for the analysis of membrane proteomes by mass spectrometry. Protein & Cell. 2012, 3(9):661-668
8. Yong Lin, Hui Liu, Zhonghua Liu,Xianchun Wang, Songping Liang. Shotgun analysis of membrane proteomes using a novel combinative strategy of solution-based sample preparation coupled with liquid chromatography-tandem mass spectrometry. J Chromatogr B, 2012, 901:18-24
9. Xianchun Wang and Songping Liang. Gel-absorption-based sample preparation method for shotgun analysis of membrane proteome. Methods Mol Biol. 2012, 869:385-392
10. Jian Zhou, Deng Bi, Yong Lin, Ping Chen, Xianchun Wang, Songping Liang. Shotgun proteomics and network analysis of ubiquitin-related proteins from human breast carcinoma epithelial cells. Mol Cell Biochem. 2012, 359: 375-384
11. Yi Liu, Yong Lin, Yizhong Yan, Jianjun Li, Quanze He, Ping Chen, Xianchun Wang, Songping Liang. Electrophoretically-driven SDS removal and protein fractionation in the shotgun analysis of membrane proteomes. Electrophoresis, 2012, 33: 316-324
12. Jian Zhou, Jianlin Li, jianjun Li , Ping Chen , Xianchun Wang X, Songping Liang S. Dried polyacrylamide gel absorption: A method for efficient elimination of the interferences from SDS-solubilized protein samples in mass spectrometry-based proteome analysis. Electrophoresis. 2010,31:3816-3822
13. Yong Lin, Yi Liu, Jianjun Li, Yan Zhao, Quanze He, Wenjun Han, Ping Chen, Xianchun Wang , Songping Liang. Evaluation and optimization of removal of an acid-insoluble surfactant for shotgun analysis of membrane proteome. Electrophoresis. 2010,31:2705-2713
14. Zhou J, Xiong J, Li J, Huang S, Zhang H, He Q, Lin Y, Chen P, Wang X, Liang S. Gelabsorption-based sample preparation for the analysis of membrane proteome by massspectrometry. Anal Biochem. 2010,404(2):204-10
15. Jian Zhou, Sha Huang, Deng Bi, Hai Zhang, Jianglin Li, Yong Lin, Ping Chen, Xianchun Wang, Songping Liang.Analysis of integral membrane proteins by heat gel-embedment combined with improved in-gel digestions. Electrophoresis, 2009,30 (23):4109-4117 16. Xiong Xia, Shen Jian-Ying, Li Jian-Jun, Zhang Hai, Wang Xian-Chun, Liang Song-Ping. Aqueous polymer two-phase partition for the proteomic analysis of plasma membrane from rat dorsal root ganglion neurons. Prog Biochem Biophys, 2009, 36(11):1458-1468 17. Xia Xiong, Sha Huang, Hai Zhang, Jian-jun Li, Jian-Ying Shen, Jixian Xiong, Yong Lin, Liping Jiang, Xian-Chun Wang, Song-Ping Liang. Enrichment and proteomic analysis of plasma membrane from rat dorsal root ganglion neurons. Proteome Sci. 2009,7:41-50 18. Yong Lin, Yan Li, Yi Liu, Wenjun Han, Quanze He, Jianglin Li, Ping Chen, Xianchun Wang , Songping Liang . Improvement of gel-separated protein identification by DMF-assisted digestion and peptide recovery after electroblotting. Electrophoresis, 2009,30(20):3626-3635 19. Zhigui Duan, Xiaojun Yan, Rui Cao, Zhen Liu, Xianchun Wang and Songping Liang.Proteomic analysis of Latrodectus tredecimguttatus venom for uncovering potential latrodectism-related proteins. J. Biochem. Mol. Toxicol. 2008,22(5):328-336 20. Yong Lin, Jian Zhou, Deng Bi, Ping Chen, Xianchun Wang , Songping Liang . Sodium deoxycholate- assisted tryptic digestion and identification of proteolytically resistant proteins. Anal Biochem. 2008, 377(2): 259-266 21.Jian Zhou, Yong Lin, Xingcan Deng, Jianying Shen, Quanyuan He, Ping Chen,Xianchun Wang,Songping Liang. Development and application of a two-phase, on-membrane digestion method in the analysis of membrane proteome. J Proteome Res. 2008, 7:1778-1783. 22.X.C. Wang, Z.G Duan, J.Yang, X.J.Yan, H. Zhou, X.Z.He, and S.P.Liang. Physiological and biochemical analysis of L. tredecimguttatus venom collected by electrical stimulation. J. Physiol. Biochem.,2007, 63(3):221-230 23. Jian Zhou,Tieyang Zhou, Rui Cao, Zhen Liu, Jianying Shen, Ping Chen, Xianchun Wang, and Songping Liang.Evaluation of the application of sodium deoxycolate to proteomic analysis of rat hippocaampal plasma membrane. J Proteome Res.2006, 5, 2547-2553 24. Duan ZG,Yan XJ,He XZ,Zhou H,Chen P,Cao R,Xiong JX,Hu WJ,Wang XC,Liang SP.Extraction and protein component analysis of venom from the dissected venom glands ofLatrodectus tredecimguttatus. Comp Biochem Physiol, Part B. 2006,145(3-4):350-357 25. Wang lin, Zhou Tieyang, Tang Xing, Wang Xianchun,Liang Songping.Purification and characterization of a novelα-amylase inhibitor from wild Amaranth (Amaranthus paniculatus)weeds. Chin J of Biochem Mol Biol, 2004,20(4):434-439 26. WANG Xian-Chun, CHEN Ping, LIANG Song-Ping. Liquid chromatography-tandem mass spectrometry analysis of a synthetic nonapeptide and its main by-products. Chin J Biochem Mol Biol, 2003,19(5):576-581
27. 段志貴,楊靜,晏曉軍,曾雄智,王賢純,梁宋平。間斑寇蛛粗毒液的生理生化分析及兩種采毒方法的比較。動(dòng)物學(xué)研究,2009,30(4):381-388
28. 楊靜,段志貴,周紅,晏曉軍,何小珍,王賢純,梁宋平。間斑寇蛛粗毒的生物學(xué)特性。動(dòng)物學(xué)報(bào),2007,53(4):682-688
29. 周鐵楊,周健,曹銳,沈建英, 陳平, 王賢純。大鼠海馬細(xì)胞質(zhì)膜的分離及其蛋白質(zhì)組學(xué)分析。中國(guó)生物化學(xué)與分子生物學(xué)報(bào),2006,22(9):733-743
30. 周鐵楊,周健,雄繼先,王賢純;诘入娋劢梗聪郒PLC的虎紋捕鳥蛛毒素組學(xué)的初步研究。中國(guó)生物化學(xué)與分子生物學(xué)報(bào),2006,22(10):823-831
31. 范春明,王賢純,梁宋平。兩種蜘蛛多肽毒素的譜法序列分析。質(zhì)譜學(xué)報(bào),2005,26(2):65-70
32. 徐峽,熊霞,李冬玲,肖玉成,王賢純,梁宋平。海南捕鳥蛛毒素-IV突變體的固相合成和生理活性分析。生物工程學(xué)報(bào),2005,21(1):92-96
33. 曹銳,張麗軍,聶松,王賢純,梁宋平。多維色譜-串聯(lián)質(zhì)譜聯(lián)用技術(shù)分離和鑒定小鼠肝臟質(zhì)膜蛋白質(zhì)。中國(guó)生物化學(xué)與分子生物學(xué)報(bào),2005,21(1):134-142
34. 王賢純,梁宋平;⒓y捕鳥蛛毒素-I Lys13突變對(duì)生物學(xué)活性的影響。自然科學(xué)進(jìn)展, 2004,14(2):227-231
35. 王賢純,梁宋平。反相高效液相色譜/電噴霧質(zhì)譜法分析化學(xué)合成七肽粗產(chǎn)物。分析化學(xué),2004,32(9):1219-1222
36. 王賢純,范春明,唐新科,陳平,梁宋平。牛血清白蛋白胰蛋白酶酶解產(chǎn)物的色譜-質(zhì)譜聯(lián)用分析及其三種數(shù)據(jù)庫(kù)搜尋鑒定方法的比較。中國(guó)生物化學(xué)與分子生物學(xué)報(bào),2004,20(3):393-398
37. 王賢純,梁宋平;瘜W(xué)合成ω-芋螺毒素MVIIA的復(fù)性與質(zhì)譜分析。動(dòng)物學(xué)研究,2003,24 (2):121-126
38. 王賢純,梁宋平。電噴霧串聯(lián)質(zhì)譜圖的疊合與多肽序列分析。生物化學(xué)與生物物理學(xué)報(bào),2001,33(6):665-670
39. 王賢純,梁宋平;瘜W(xué)合成虎紋捕鳥蛛毒素-I的色譜行為及分離純化。色譜,2001,19(2):101-104
40. 王賢純,梁宋平,羅澤民。虎紋捕鳥蛛毒素-I單殘基突變體R20A-HWTX-I的化學(xué)合成與性質(zhì)分析。生物工程學(xué)報(bào),2000,16(4):490-494
41. 王賢純,梁宋平,羅澤民。構(gòu)建同時(shí)攜帶HWTX-I和AAI活性位點(diǎn)的多肽嵌合體。生物化學(xué)與生物物理學(xué)報(bào),2000,32(3):274-280
42. 王賢純,梁宋平,羅澤民;⒓y捕鳥蛛毒素-I突變體A1Y-HWTX-I的固相合成和生物學(xué)活性測(cè)定。中國(guó)生物化學(xué)與分子生物學(xué)報(bào),2000,16(3):357-362
43. 王賢純,梁宋平。不同溶劑對(duì)合成多肽的洗脫效果比較;瘜W(xué)通報(bào)(網(wǎng)絡(luò)版),1999,c00083
44. 王賢純,謝錦云,單茶秀,戚志仁。姜黃的綜合利用—水蒸汽蒸餾法提取姜黃油。食品科學(xué),1993,8:49-51
45. 王賢純,謝錦云,單茶秀,戚志仁。姜黃的綜合利用—乙醇循環(huán)法提取姜黃素。食品科學(xué),1993,9:45-47
46. 王賢純。姜黃色素的穩(wěn)定性。食品與發(fā)酵工業(yè),1994,1:63-66
47. 王賢純, 陳 平,王 迎, 王美遲, 曾雄智, 梁宋平。生物化學(xué)雙語(yǔ)教學(xué)的課堂實(shí)踐與探索。教學(xué)研究,2013,36(5):57-60
48. 王賢純,陳 平,王 迎,王美遲,曾雄智,段志貴,劉 珍,梁宋平。生物化學(xué)雙語(yǔ)教材建設(shè)的探討。高師理科學(xué)刊,2012,32(1):101-103
49. 王賢純, 陳 平, 王 迎,曾雄智, 王美遲,梁宋平。關(guān)于進(jìn)一步推進(jìn)高校本科雙語(yǔ)教學(xué)的幾點(diǎn)思考。高等教育研究學(xué)報(bào),2008,31(2):36-37
50. 王賢純, 陳 平, 曾雄智, 王美遲。 高校雙語(yǔ)教學(xué)的實(shí)施策略。北京大學(xué)學(xué)報(bào)(哲學(xué)社會(huì)科學(xué)版),2007,S2:68-69