柴人杰，男，浙江寧波人，1982年11月出生。2004年在中國(guó)科學(xué)技術(shù)大學(xué)獲生物科學(xué)專業(yè)學(xué)士學(xué)位，2009年獲美國(guó)貝勒大學(xué)（Baylor University）生物醫(yī)學(xué)專業(yè)博士學(xué)位。

2009年至2013年于斯坦福大學(xué)（Stanford University）醫(yī)學(xué)院從事博士后研究工作。博士后期間作為項(xiàng)目負(fù)責(zé)人主持的課題獲美國(guó)斯坦福大學(xué)醫(yī)學(xué)院院長(zhǎng)獎(jiǎng)勵(lì)基金和美國(guó)聽覺健康基金會(huì)資助，并兩次獲斯坦福大學(xué)醫(yī)學(xué)院耳鼻喉系（第2屆和第3屆）研究成果討論會(huì)優(yōu)秀研究成果獎(jiǎng)，兩次獲美國(guó)耳鼻喉研究學(xué)會(huì)（第34屆和第35屆）年會(huì)博士后旅行獎(jiǎng)金。

2013年8月受聘東南大學(xué)生命科學(xué)研究院教授，博士生導(dǎo)師。

2014年獲國(guó)際內(nèi)耳研究學(xué)會(huì)第57屆年會(huì)優(yōu)秀青年研究者旅行獎(jiǎng)金。

2015年6月獲江蘇省杰出青年科學(xué)基金資助，2015年8月獲江蘇省“雙創(chuàng)計(jì)劃”資助，2016年8月獲國(guó)家優(yōu)秀青年科學(xué)基金資助.

此外，柴人杰教授還獲得科技部青年973計(jì)劃，國(guó)家自然科學(xué)基金委面上項(xiàng)目，人社部留學(xué)人員科技活動(dòng)重點(diǎn)項(xiàng)目，教育部留學(xué)人員項(xiàng)目，國(guó)家自然科學(xué)基金委青年項(xiàng)目，江蘇省自然科學(xué)基金委青年項(xiàng)目，江蘇省六大人才高峰高層次人才選拔培養(yǎng)計(jì)劃，東南大學(xué)優(yōu)秀青年教師教學(xué)科研資助計(jì)劃，東南大學(xué)優(yōu)青培育計(jì)劃等研究項(xiàng)目的資助。

內(nèi)耳干細(xì)胞調(diào)控機(jī)制的研究

聽力障礙是一種常見的耳疾。根據(jù)WHO 1985年全球聽力殘疾人數(shù)為4200萬，1995年估計(jì)為1.2億，2001年上升到2.5億，2005年達(dá)到2.78億，占全球人口的4.6%，其中80%殘疾人群生活在中低收入國(guó)家。其中老年性耳聾的主要原因是由于內(nèi)耳毛細(xì)胞和螺旋神經(jīng)元的損傷，而永久性感音神經(jīng)性聾主要是由于內(nèi)耳毛細(xì)胞的不可逆損傷和由于毛細(xì)胞損傷而導(dǎo)致的螺旋神經(jīng)元神經(jīng)突的退化造成的。因此如何使內(nèi)耳毛細(xì)胞和螺旋神經(jīng)元神經(jīng)突在損傷和丟失后修復(fù)和再生，是近年來聽覺領(lǐng)域研究的重點(diǎn)。本實(shí)驗(yàn)室的研究方向主要著眼于Wnt信號(hào)通路對(duì)內(nèi)耳干細(xì)胞的調(diào)控。通過對(duì)于內(nèi)耳干細(xì)胞調(diào)控機(jī)制的研究，誘導(dǎo)這些干細(xì)胞增殖分化成為具有功能的耳蝸毛細(xì)胞和螺旋神經(jīng)元細(xì)胞，從而治療聽力障礙。

近5年來發(fā)表SCI論文25篇，其中第一作者6篇，通訊作者2篇，另有國(guó)際會(huì)議大會(huì)發(fā)言2篇，國(guó)際會(huì)議論文22篇。

近期代表性論文

X. Wang#, X. Wang#, Yuan W, R.J. Chai*（并列通訊作者）, D. Liu*. Egfl6 is involved in zebrafish notochord development. Fish Physiol Biochem. 2015 May 8,doi: 10.1007/s10695-015-0061-x

T. Wang#, R.J. Chai#（并列第一作者）,G. Kim, .X. Pham, L. Jansson, D. Nguyen, B. Kuo, L. May, E. Rubel, J. Zuo, L. Cunningham, A.G. Cheng*. Damage-Recruited Lgr5+ Cells Regenerate Hair Cells via Proliferation and Direct Transdifferentiationin Neonatal Mouse Utricle. Nature Communications, 2015 Apr 7;6:6613. doi: 10.1038/ncomms7613.

S. Sun, M. Sun, Y. Zhang, C. Cheng, M. Waqas, H. Yu, Y. He, B. Xu, S. Yin, R.J. Chai*（并列通訊作者）, H. Li*. In vivo overexpression of X-linked inhibitor of apoptosis protein protects against neomycin-induced hair cell loss in the apical turn of the cochlea during the ototoxic-sensitive period. <a href=#, R.J. Chai#（并列第一作者）, A. Lenoir, Z. Liu, L. Zhang, K. Steigelman, J. Fang, R. Palmiter, E. Rubel, A.G. Cheng*, J. Zuo*. Spontaneous hair cell regeneration in the neonatal mouse cochlea in vivo. Development, 141(4):816-29. doi:10.1242/dev.103036. 2014

T.A. Jan#, R.J. Chai#（并列第一作者）, Z. Sayyid, R. van Amerongen, A. Xia, T. Wang T, S.T. Sinkkonen, J.R. Levin, Y. Zheng, S. Heller, R. Nusse*, A.G. Cheng*. Tympanic border cells are Wnt-responsive and act as progenitors for postnatal mouse cochlear cells. Development 140, 1196-1206, doi:10.1242/dev.087528. 2013

R.J. Chai, B. Kuo, T. Wang, E. J. Liaw, A. Xia, T. A. Jan, Z. Liu, M. M. Taketod, J. S. Oghalaia, R. Nussec, J. Zuo, and A. G. Cheng*.Wnt signaling induces proliferation of sensory precursors in the postnatal mouse cochlea.Proc Natl Acad Sci U S A. 109(21):8167-72. doi: 10.1073/pnas.1202774109. 2012 May 22

R.J. Chai, A. Xia, T. Jan, T. Hayashi, O. Bermingham-McDonogh, A.G. Cheng*. Dynamic expression of Lgr5, a Wnt target gene, in the developing and mature mouse inner ear. Journal of the Association for Research in Otolaryngology (JARO); 12(4):455-69. PMCID: PMC3123443. 2011 Aug

R.J. Chai, S.Y Chen, J.H. Ding*, P. Grayburn*. Efficient, Glucose Responsive, and Islet-Specific Transgene Expression by a Modified Rat Insulin Promoter. Gene Therapy 16, 1202u20131209 PMCID: PMC2762485. 2009

Y. He , D. Tang , C. Cai , R.J. Chai, H. Li*. LSD1 is Required for Hair Cell Regeneration in Zebrafish.Molecular Neurobiology 2015 May 26. [Epub ahead of print]

H. Mei, S.Sun, Y.Bai, Y. Y. Chen , R.J. Chai, H. Li*. Reduced mtDNA copy number increases the sensitivity of tumor cells to chemotherapeutic drugs.Cell Death & Disease2015 Apr 2;6:e1710. doi: 10.1038/cddis.2015.78.

R. Feng, Q. Sang, Y.Zhu, W. Fu, M. Liu, Y. Xu, H. Shi, Y.Xu, R. Qu, R.J. Chai,R. Shao, L.Jin, L. He, X.Sun, L. Wang*.MiRNA-320 in the human follicular fluid is associated with embryo quality in vivo and affects mouse embryonic development in vitro. Scientific Report 2015 Mar 3;5:8689. doi: 10.1038/srep08689

W. Li, J. Wu, J. Yang, S. Sun, R.J. Chai, Z. Chen* , H. Li*Notch inhibition inducesmitoticallygenerated hair cells in mammalian cochleae via activating the Wnt pathway. Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):166-71. doi: 10.1073/pnas.1415901112

Y. Chen, L. Li, W. Ni, Y. Zhang, S. Sun, D. Miao, R.J. Chai, H. Li*Bmi1 regulates auditory hair cell survival by maintaining redox balance.Cell Death & Disease 2015 Jan 22;6:e1605. doi: 10.1038/cddis.2014.549.

S. Sun, H. Yu, H. Mei, W. Ni, Y. Zhang, L. Guo, Y. He, Z.Xue, Y.Ni, J. Li, Y. Feng, Y.Chen, R. Shao, R.J. Chai,H. Li*.Inhibition of the Activation and Recruitment ofMicroglia-Like Cells Protects AgainstNeomycin-Induced Ototoxicity.Molecular Neurobiology 2015，51（1）：252-267PMID: 24781382

D. Chang, R.J. Chai, R. DiMarco, S. Heilshorn, A.G. Cheng*. Protein-Engineered Hydrogel Encapsulation for 3D Culture of Murine Cochlea. Otology & Neurotology, 2015，36（3）：531-538

Q. Sang, J. Zhang, R. Feng, X. Wang ,Q.Li ,X.Zhao,Q.Xing,Q.Chen, J. Du,S.Sun, R.J. Chai,, L. Jin, L. He ,H.Li* ,L.Wang*. Ildr1b is essential for semicircular canal development, migration of the posterior lateral line primordium, and hearing ability in zebrafish: Implications for a role in the recessive hearing impairment DFNB42.Human Molecular Genetic.2014 Dec 1;23(23):6201-11. doi: 10.1093/hmg/ddu340.

W. Yan , J. Li , R.J. Chai,W. Guo , L. Xu , Y. Han , X.Bai , H.Wang*.Combining Use of Captopril and Losartan Attenuates the Progress of Streptococcus pneumoniae-Induced Tympanosclerosis through the Suppression of TGF-β1 Expression.PLoS One. 2014 Oct 31;9(10):e111620. doi: 10.1371/journal.pone.0111620.

X. Bai, H. Lv, H. Zhang, J. Liu, Z.Fan, L.Xu, Y. Han, R.J. Chai,J. Li, H. Wang.Identification of a novel missense mutation in the WFS1 gene as a cause of autosomal dominant nonsyndromic sensorineural hearing loss in all-frequencies.Am J Med Genet A. 2014 Sep 23. doi: 10.1002/ajmg.a.36760. [Epub ahead of print]

Y. He, H. Yu, C. Cai, S. Sun, H, R.J. Chai,H. Li*Inhibition of H3K4me2 Demethylation Protects Auditory Hair Cells from Neomycin-Induced Apoptosis. Molecular Neurobiology 2014 Aug 19. 10.1007

X. Gu, L. Guo, H. Ji, S. Sun, R.J. Chai, L. Wang, H. Li*. Genetic testing for sporadic hearing loss using targeted massively parallel sequencing identifies 10 novel mutations. Clinical Genetics2014 May 22. doi: 10.1111/cge.12431

S. Volkenstein, K. Oshima, S. T. Sinkkonen, C. Corrales, S. Most, R.J. Chai, T. Jan, A.G. Cheng, S. Heller*. Transient, afferent input-dependent, postnatal niche for neural progenitor cells in the cochlear nucleus. Proc Natl Acad Sci U S A. 2013 Aug 27, 110(35):14456-61. doi: 10.1073/pnas.1307376110.

A. Vu, J. Kim, M. Huth, L. Luk, R.J. Chai, A.J. Ricci, A. G. Cheng*. Integrity and regeneration of mechanotransduction machinery regulates aminoglycoside entry and sensory cell death Plos One, 2013, 8(1):e54794. PMCID:PMC3554584.

S. T. Sinkkonen, R.J. Chai, T. Jan, R. D. Laske, A.G. Cheng, K. Oshima, S. Heller*.Intrinsic regenerative potential of murine cochlear supporting cells Scientific Report2011 1, 26; DOI:10.1038/srep00026.

T. Jan, R.J. Chai, Z. Sayyid, A.G. Cheng*. Isolating LacZ-expressing cells from mouse inner ear tissues using flow cytometry. J Vis Exp, (58). 2011 Dec 23;