，男，1982.4.29出生，職 稱 教授。

以維生素C和苯丙素類化合物為目標(biāo)產(chǎn)物，闡明微生物高效異源合成植物天然產(chǎn)物的生理機制，并通過計算機輔助的代謝途徑設(shè)計、代謝途徑單元模塊化調(diào)控、輔因子平衡與再生調(diào)控、反義RNA工程等策略，在微生物中實現(xiàn)來源于植物的高附加值天然產(chǎn)物的高效異源合成。

前五年在Biotechnology Advances、Metabolic Engineering、Eukaryotic Cell和Applied and Environmental Microbiology等生物工程和微生物生理學(xué)權(quán)威學(xué)術(shù)期刊上發(fā)表與本項目相關(guān)的研究論文和綜述45篇，授權(quán)發(fā)明專利14項，參編發(fā)酵工程專業(yè)著作4本。

代表性著作和論文如下:

[1] Saijie Zhu, Junjun Wu, Guocheng Du,Jingwen Zhou*, Jian Chen. Efficient synthesis of eriodictyol from L-tyrosine inEscherichia coli.Applied and Environmental Microbiology. 2014, 10(80): 3072-3080.(2012 IF= 3.678)

[2] Lili Gao, Yudong Hu, Jie Liu, Guocheng Du,Jingwen Zhou*, Jian Chen. Stepwise metabolic engineering ofGluconobacter oxydansWSH-003 for the direct production of 2-keto-L-gulonic acid from D-sorbitol.Metabolic Engineering. 2014, 24: 30u201337.(2012 IF=6.859)

[3] Shaohui Zhao, Xinrui Zhao, Huijun Zou, Jianwei Fu, Jian Chen,Jingwen Zhou, Guocheng Du. Comparative proteome analysis ofSaccharomyces cerevisiaegrown under different nitrogen source conditions.Journal of Proteomics.2014, 101: 102-112(2012 IF=4.088)

[4]Jingwen Zhou, Guocheng Du, Jian Chen. Novel fermentation processes for manufacturing plant natural products.Current Opinion in Biotechnology. 2014. 25: 17-23.(2012 IF= 7.86)

[5] Xinrui Zhao, Guocheng Du, Huijun Zou, Jianwei Fu,Jingwen Zhou, Jian Chen. Metabolic engineering of the regulators in nitrogen catabolite repression to reduce the production of ethyl carbamate in a model rice wine system.Applied and Environmental Microbiology. 2014, 80(1): 392-398.(2012 IF= 3.678)

[6] Lili Gao, Jie Liu, Guocheng Du,Jingwen Zhou, Jian Chen. Characterization of a group of pyrroloquinoline quinone-dependent dehydrogenases that are involved in the conversion of L-sorbose to 2-Keto-L-gulonic acid inKetogulonicigenium vulgareWSH-001.Biotechnology Progress. 2013. 29(6):1398-1404. (2012 IF= 1.853)

[7] Jidong Liu, Weiping Zhang, Guocheng Du, Jian Chen,Jingwen Zhou. Overproduction of geraniol by enhanced precursor supply inSaccharomyces cerevisiae.Journal of Biotechnology.2013, 168(4): 446-451.(2012 IF= 3.183)

[8] Xinrui Zhao, Huijun Zou, Jianwei Fu,Jingwen Zhou, Jian Chen, Guocheng Du. Nitrogen regulation involved in the accumulation of urea inSaccharomyces cerevisiaeused in rice wine production.Yeast. 2013. 30(11): 437-447.(2012 IF= 1.955)

[9] Junjun Wu, Peiran Liu, Yongming Fan, Han Bao, Guocheng Du,Jingwen Zhou, Jian Chen. Multivariate modular metabolic engineering ofEscherichia colito produce resveratrol from L-tyrosine.Journal of Biotechnology. 2013, 167: 404-411.(2012 IF= 3.183)

[10]Jingwen Zhou, Qiaoshuang Zheng, Jie Liu, Jianmin Huang, Guocheng Du, Jian Chen. Indigenous plasmids ofBacillus megateriumWSH-002 involved in mutualism withKetogulonicigenium vulgareWSH-001.Plasmid.2013, 70(2):240-246.(2012 IF=1.276)

[11] Xinrui Zhao, Guocheng Du, Huijun Zou, Jianwei Fu,Jingwen Zhou*, Jian Chen. Progress in preventing the accumulation of ethyl carbamate in alcoholic beverages.Trends in Food Science and Technology.2013, 32: 97-107.(2012 IF=4.135)

[12] Xiaobei Wang, Jie Liu, Guocheng Du,Jingwen Zhou, Jian Chen. Efficient production of L-sorbose from D-sorbitol by whole cell immobilization ofGluconobacter oxydansWSH-003.Biochemical Engineering Journal.2013, 77: 171u2013176.(2012 IF=2.579)

[13] Jidong Liu, Yibo Zhu, Guocheng Du,Jingwen Zhou, Jian Chen. Response ofSaccharomyces cerevisiaeto D-limonene-induced oxidative stress.Applied Microbiology and Biotechnology. 2013, 97(14): 6467-6475.(2012 IF=3.689)

[14] Yongkun Lv, Xinrui Zhao, Long Liu, Guocheng Du,Jingwen Zhou*, Jian Chen. A simple procedure for protein ubiquitination detection inSaccharomyces cerevisiae: Gap1p as an example.Journal of Microbiological Methods.2013, 94: 25-29.(2012 IF=2.161)

[15]Jingwen Zhou, Zhenghong Xu, Shouwen Chen. Simulation and prediction of the thuringiensin abiotic degradation processes in aqueous solution by a radius basis function neural network model.Chemosphpere.2013. 91(4): 442-447.(2012 IF=3.137)

[16] Shimon Bershtein, Wanmeng Mu, Adrian Serohijos,Jingwen Zhou, Eugene I. Shakhnovich. Protein quality control shapes the fitness effects of mutations inE. coli.Molecular Cell.2013. 49(1): 133-144.(2012 IF=15.28)

[17] Jidong Liu, Yibo Zhu, Guocheng Du,Jingwen Zhou, Jian Chen. Exogenous ergosterol protectsSaccharomyces cerevisiaefrom D-Limonene stress.Journal of Applied Microbiology. 2013. 114(2):482-491.(2012 IF=2.196)

[18] Junjun Wu, Guocheng Du,Jingwen Zhou, Jian Chen.Metabolic engineering ofEscherichia colifor (2S)-pinocembrin production from glucose by a modular metabolic strategy.Metabolic Engineering.2013. 16:48-55.(2012 IF=6.859)

[19] Hua Liu, Guocheng Du, Jianghua Li,Jingwen Zhou, Jian Chen. Enhanced production of a-cyclodextrin glycosyltransferase inEscherichia coliby codon usage optimization.Journal of Industrial Microbiology & Biotechnology.2012. 39(12): 1841-1849.(2012 IF=2.321)

[20] Xiaoxia Yin, Catherine Madzak, Guocheng Du,Jingwen Zhou, Jian Chen. Enhanced a-ketoglutarate production inYarrowialipolyticaWSH-Z06 by regulation of the pyruvate carboxylation pathway.Applied Microbiology and Biotechnology. 2012. 96(6): 1527-1537.(2012 IF=3.689)

[21]Jingwen Zhou, Hua Liu, Jianghua Li, Guocheng Du, Jian Chen. Production of a-cyclodextrin glycosyltransferase inBacillus megateriumMS941 by systematic codon usage optimization.Journal of Agricultural and Food Chemistry.2012, 60(41), 10285u201310292.(2012 IF=2.906)

[22]Jingwen Zhou, Xiaoxia Yin,CatherineMadzak, Guocheng Du, Jian Chen. Enhanceda-ketoglutarate production inYarrowialipolyticaWSH-Z06 by alteration of the acetyl-CoA metabolism.Journal of Biotechnology. 2012.161: 257-264.(2012 IF= 3.183)

[23] Feifei Hu, Jidong Liu, Guocheng Du, Zhaozhe Hua,Jingwen Zhou, Jian Chen. The key cytomembrane ABC transporters ofSaccharomyces cerevisiaefailed to improve the tolerance to D-limonene.Biotechnology Letters.2012. 34(8): 1505-1509.(2012 IF=1.853)

[24] Lili Gao,Jingwen Zhou, Jie Liu, Guocheng Du, Jian Chen. Draft Genome Sequence ofGluconobacter oxydansWSH-003, an Extremely Saccharide and Alditol-Tolerant Strain.Journal of Bacteriology.2012. 194(16) 4455-4456. (2012 IF=3.194)

[25] Yibo Zhu, Jie Liu, Guocheng Du,Jingwen Zhou, Jian Chen. A high throughput method to screen companion bacterium for 2-keto-L-gulonic acid biosynthesis by co-cultureKetogulonigenium vulgare.Process Biochemistry.2012. 47: 1428-1432.(2012 IF=2.414).

[26] Zongzhong Yu, Guocheng Du,Jingwen Zhou, Jian Chen. Enhanceda-ketoglutaric acid production inYarrowia lipolyticaWSH-Z06by an improved integrated fed-batch strategy.Bioresource Technology. 2012. 114: 597-602.(2012 IF=4.75)

[27] Yibo Zhu, Jie Liu, Guocheng Du,Jingwen Zhou, Jian Chen. Sporulation and stable spore ofBacillus megateriumenhanceKetogulonigenium vulgarepropagation and 2-keto-L-gulonic acidbiosynthesis.Bioresource Technology.2012. 107: 399-404.(2012 IF=4.75)

[28] Zhengxiong Zhou, Guocheng Du, Zhaozhe Hua,Jingwen Zhou, Jian Chen. Optimization of fumaric acid production byRhizopus delemarbased on the morphology formation.Bioresource Technology. 2011, 102: 9345-9349.(2012 IF=4.75)

[29]Jingwen Zhou, Liming Liu, Jian Chen. Improved ATP supply enhances acid tolerance ofCandida glabrataduring pyruvic acid production.Journal of Applied Microbiology. 2011, 110(1):44-53.(2012 IF=2.196)

[30]Jingwen Zhou, Liming Liu, Jian Chen. Method to purify mitochondrial DNA directly from yeast total DNA.Plasmid. 2010, 64(3):196-199.(2012 IF=1.276)

[31]Jingwen Zhou, Haiyan Zhou, Guocheng Du, Liming Liu, Jian Chen. Screening of a thiamine auxotrophic yeast for α-ketoglutaric acid overproduction.Letters in Applied Microbiology. 2010, 51(3):264-271.(2012 IF=1.629)

[32]Jingwen Zhou, Liming Liu, Jian Chen. Mitochondrial DNA heteroplasmy inCandida glabrataafter mitochondrial transformation.Eukaryotic Cell. 2010, 9(5): 806-814.(2012 IF=3.586)

[33]Jingwen Zhou, Liming Liu, Zhongping Shi, Guocheng Du, Jian Chen.ATP in Current Biotechnology: Regulation, Application and Perspectives.Biotechnology Advances.2009, 27(1): 94-101.(2012 IF=9.599)

[34]Jingwen Zhou, Luxi Huang, Liming Liu Jian Chen. Enhancement of pyruvate productivity by inducible expression of a F0F1-ATPase inhibitorINH1 in Torulopsis glabrataCCTCC M202019.Journal of Biotechnology. 2009, 144(2): 120-126.(2012 IF= 3.183)

[35]Jingwen Zhou, Zhiyao Dong, Liming Liu, Guocheng Du, Jian Chen.A reusable method for construction of non-marker large fragment deletion yeast auxotroph strains: a practice inTorulopsis glabrata.Journal of Microbiological Methods.2009, 76(1): 70u201374.(2012 IF=2.161)

[36]Jingwen Zhou, Yafei Chang, Zhenghong Xu, Ziniu Yu, Shouwen Chen. Production of thuringiensin by fed-batch culture ofBacillus thuringiensissubsp.darmstadiensis032 with an improved pH-control glucose feeding strategy,Process Biochemistry. 2007, 42(1): 52-56.(2012 IF=2.414)

[37] 郭洪偉, 堵國成, 陳堅,周景文*. 利用微生物發(fā)酵生產(chǎn)a-酮戊二酸進展.生物工程學(xué)報. 2013, 29(2): 141?152.

[38] 孫明雪, 劉繼棟, 堵國成,周景文*, 陳堅. 調(diào)控釀酒酵母類異戊二烯合成途徑強化芳樟醇合成.生物工程學(xué)報. 2013, 29(6): 751?759.

[39] 殷曉霞, 堵國成, 陳堅,周景文*. 強化乙酰輔酶A供給對解脂亞洛酵母產(chǎn)a-酮戊二酸的影響.工業(yè)微生物. 2013, 43(2): 45-50.

[40] 余宗鐘, 堵國成, 陳堅,周景文*. 基于細(xì)胞代謝調(diào)控的a-酮戊二酸發(fā)酵過程放大.工業(yè)微生物. 2013, 43(3): 23-28.

[41]周景文, 董志姚, 劉立明, 堵國成, 陳堅. 葡萄糖酸鈉對光滑球擬酵母CCTCC M202019能量代謝和丙酮酸積累的影響.華中農(nóng)業(yè)大學(xué)學(xué)報(自然科學(xué)版), 2010, 29(4): 10-15.

[42]周景文, 徐建, 陳守文, 喻子牛. 基于徑向基神經(jīng)網(wǎng)絡(luò)和遺傳算法的聚-γ-谷氨酸發(fā)酵培養(yǎng)基優(yōu)化.食品科學(xué). 2006, 27(10): 270-274.

[43]周景文，堵國成，陳堅*. 發(fā)酵食品有害氨(胺)類代謝物：形成機制和消除策略.中國食品學(xué)報. 2011, 9: 8-25.

[44] 朱益波,周景文*，陳堅. 巨大芽孢桿菌與普通生酮基古龍酸菌共培養(yǎng)過程中相互作用分子機制研究進展.微生物學(xué)報. 2012, 52(8):940-947.

[45] 張偉平, 趙鑫銳, 堵國成, 鄒慧君, 傅建偉,周景文*, 陳堅. 釀酒酵母氮代謝物阻遏效應(yīng)及其對發(fā)酵食品安全的影響.應(yīng)用與環(huán)境生物技術(shù)學(xué)報. 2012, 18(05): 862-872.

*:通訊作者。

前五年主持或完成國家自然科學(xué)基金、江蘇省自然科學(xué)基金和國家/部級重點實驗室開放課題及企業(yè)橫向委托項目8項。其中代表性的科研項目如下:

[1]解脂亞洛酵母酮酸轉(zhuǎn)運蛋白的生理功能與調(diào)控機制. 國家自然科學(xué)基金面上項目(21276109). 2013年1月1日-2016年12月1日.項目主持人；

[2]微生物高效積累黃酮類化合物的關(guān)鍵共性問題研究.江南大學(xué)自主科研計劃-重點項目(JUSRP51307A), 2013年1月-2015年12月.項目主持人；

[3]維生素C合成過程吡咯喹啉醌代謝關(guān)鍵基因及其調(diào)控機制. 教育部新世紀(jì)優(yōu)秀人才支持計劃(NCET-12-0876), 2012年1月-2015年12月.項目主持人；

[4]維生素C從頭合成關(guān)鍵模塊功能解析與調(diào)控，高等學(xué)校全國優(yōu)秀博士學(xué)位論文作者專項資金(2011046)，2012年1月-2016年12月.項目主持人；

[5]生化網(wǎng)絡(luò)重構(gòu)與維生素生產(chǎn)新菌種.863項目(2012AA022103).2012年11月1日-2015年12月31日；

[6]食品安全全程控制的食品制造過程設(shè)計與構(gòu)建.國家重點基礎(chǔ)研究發(fā)展計劃(973計劃，2012CB720806).2012年1月-2016年8月.子課題負(fù)責(zé)人；

[7]代謝工程改造釀酒酵母合成D-檸檬烯的關(guān)鍵問題研究. 國家自然科學(xué)基金青年科學(xué)基金項目 (31000807). 2011年1月1日-2013年12月1日.項目主持人；

[8]代謝工程改造釀酒酵母合成S-芳樟醇及其發(fā)酵過程優(yōu)化. 江蘇省基礎(chǔ)研究計劃(BK201020722). 2010年 7月1日-2013年7月1日.項目主持人。

[1] 2013年12月，新型有機酸的發(fā)酵法制備技術(shù)，陳堅、周景文、劉龍、李江華、劉立明，2013年中國輕工業(yè)聯(lián)合會技術(shù)發(fā)明獎一等獎；

[2] 2012年8月，發(fā)酵法生產(chǎn)α-酮戊二酸，堵國成、周景文、李江華、陳堅、宗偉剛、胡志杰、王立軍，2012年無錫市科技進步獎二等獎；

[3] 2011年11月，光滑球擬酵母中ATP的生理功能與作用機制，周景文，全國百篇優(yōu)秀博士學(xué)位論文(證書編號：2011046)；

[4] 2011年10月，《發(fā)酵過程優(yōu)化原理與技術(shù)》，陳堅、堵國成、劉立明、周景文、劉龍等，中國石油和化學(xué)工業(yè)聯(lián)合會，科技進步獎一等獎(證書編號：2011JBR0516-1-6)；

[5] 2011年2月，工業(yè)微生物生理特性與代謝功能研究，陳堅、堵國成、劉立明、吳敬、周景文、張娟、劉龍、張東旭，江蘇省科技技術(shù)獎一等獎(證書編號：2010-1-3-R-5)；

[6] 2010年10月，光滑球擬酵母中ATP的生理功能與作用機制，周景文，江蘇省2010年度優(yōu)秀博士論文(證書編號：CX10B064)。