個(gè)人信息
王江云，研究員。
個(gè)人經(jīng)歷
2007-current 生物物理所結(jié)構(gòu)生物學(xué)與分子生物學(xué)研究中心 研究員
2003-2007 博士后, Scripps 研究所
1998-2003 化學(xué)博士, Univ. of Illinois at Urbana Champaign
1994-1998 化學(xué)物理學(xué)士, 中國科技大學(xué)少年班零零班
通過進(jìn)化tRNA和tRNA synthetase, 我擴(kuò)展了基因密碼, 使得在活細(xì)胞中的TAG停止密碼子可以編碼熒光氨基酸,有化學(xué)反應(yīng)性的氨基酸和羥基酸. 這些技術(shù)使得我們可以在活細(xì)胞中定位不能用綠色熒光蛋白定位的蛋白質(zhì); 制備脂化或糖化的蛋白質(zhì); 并且研究蛋白折疊和酶學(xué)。 同時(shí)通過從頭設(shè)計(jì)的方法, 我也設(shè)計(jì)了含有heme的蛋白質(zhì), 并且用NMR的方法測定了結(jié)構(gòu)。
在研項(xiàng)目
科技部973 “表觀遺傳學(xué)的化學(xué)生物學(xué)研究”，任課題組長。
科技部973 “基因密碼子的分子生物學(xué)研究”，任課題骨干。
中國科學(xué)院重點(diǎn)部署項(xiàng)目 “非編碼RNA”，任項(xiàng)目首席。
國家自然科學(xué)基金委 “杰出青年基金”，任課題負(fù)責(zé)人。
國家自然科學(xué)基金委“重大研究計(jì)劃培育項(xiàng)目”，任課題負(fù)責(zé)人
代表論文
1. Lv, X. X.; Yu, Y.; Zhou, M.; Hu, C.; Gao, F.; Li, J. S.; Liu, X. H.; Deng, K.; Zheng, P.; Gong, W. M.; Xia, A. D.; Wang, J. Y.* Ultrafast Photo-induced Electron Transfer in Green Fluorescent Protein Bearing a Genetically Encoded Electron Acceptor
2. Yu, Y.; Zhou, Q.; Wang, L;.Liu, X.; Zhang, W.; Hu, M. ; Dong, J. ; Li, J.; Lv, X.; Ouyang, H.; Li, H.; Gao, F; Gong, .W.; Lu, Y.; Wang, J. Y*, Significant Improvement of Oxidase Activity through the Genetic Incorporation of a Redox-active Unnatural Amino Acid
3. Yu, Y.; Lv, X. X.; Li, J. S.; Zhou, Q.; Cui, C.; Hosseinzadeh, P.; Mukherjee, A.; Nilges, M. J.; Wang, J. Y.* and Lu, Y.* Defining the Role of Tyrosine and Rational Tuning of Oxidase Activity by Genetic Incorporation of Unnatural Tyrosine Analogs
4. Li, F. H.; Dong, J. S.; Hu, X.; Gong, W. M.; Li, J.S.; Shen, J.; Tian, H.; and Wang, J. Y. * A Covalent Approach for Site-Specific RNA Labeling in Mammalian Cells
5. Liu, X. H.; Jiang, L..; Li, J.; Wang, L.; Yu, Y.; Zhou, Q.; Lv, X. X.; Gong, W. M.; Lu, Y.; Wang, J.Y. *. Significant Expansion of Fluorescent Protein Sensing Ability through the Genetic Incorporation of Superior Photo-Induced Electron-Transfer Quenchers
6. Pan, Y.; Jin, J.; Yu, Y.; Wang, J. Y.*. Significant Enhancement of hPrx1 Chaperone Activity through Lysine Acetylation. ChemBioChem
7. Hu, C; Chan, S.; Sawyer, E. B.; Yu, Y.; Wang, J. Y. *. Metalloprotein design using genetic code expansion
8. Wu, F.C.; Zhang, H; Zhou, Q.; Wu, M; Ballard, Z; Tian, Y ; Wang, J. Y. ; Niu, ZW; Huang, Y. Expanding the genetic code for site-specific labelling of tobacco mosaic virus coat protein and building biotin-functionalized virus-like particles
9. Li,F. H.; Zhang, H.; Sun, Y.; Pan, Y. C. ; Zhou, J. Z.; J. Y. Wang* Expanding the Genetic Code for Photoclick Chemistry in E. coli, Mammalian Cells, and A
10. Li, F. H., Shi P., Li J. S.,Yang F., Wang T. Y., Zhang W., Gao F., Ding W., Li D., Li J., Xiong Y., Sun J. P., Gong W. M.*, Tian C. L.*, Wang J. Y.*A Genetically Encoded 19F NMR Sensor for Tyrosine Phosphorylation
11. Liu, X. H., Li J. S., Wang J. Y.*Significant Expansion of the Fluorescent Protein Chromophore through the Genetic Incorporation of an Unnatural Amino Acid
12. Zhou Q., Hu M. R., Zhang W., Jiang, L., Perrett S., Zhou J., Wang J. Y.*Probing the Function of the Tyr-Cys Crosslink in Metalloenzymes through the Genetic Incorporation of 3-Methylthiotyrosine
13. Liu, X. H., Yu, Y., Hu, C., Zhang, W., Lu, Y.*, Wang J. Y.*Significant Increase of Oxidase Activity through the Genetic Incorporation of a Tyrosine-Histidine Cross-Link in a Myoglobin Model of Heme-Copper Oxidase
14. Liu, X. H., Li, J. S., Dong, J. S., Hu , C., Gong, W. M.*, Wang, J. Y.* Genetic Incorporation of a Metal Chelating Amino Acid as a Probe for Protein Electron Transfer. Angew. Chem. Intl. Ed
15. Yu, Z., Pan, Y. C., Wang. Z. Y., Wang, J. Y.*, Lin. Q.*Genetically Encoded Cyclopropene Directs Rapid, Photoclick Chemistry- Mediated Protein Labeling in Mammalian Cells
16. Wang, J. Y.*, Zhang, W., Song, W. J., Wang, Y. Z., Yu, Z. P., Li, J. S., Wu, M. H., Wang, L., Zang, J. Y., Lin, Q.*A Biosynthetic Route to Photoclick Chemistry on Proteins